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GFP anticorps

Name: GFP anticorps
SKU: ABIN1742293
Price: 522.46 EUR
Availability: InStock

GFP Reactivité: Aequorea victoria WB, ICC Hôte: Lapin Polyclonal unconjugated

dilution: 1 : 1000, sample: total cell lysate of GFP transfected fibroblasts (GFP anticorps)

Image no. 1 for anti-Green Fluorescent Protein (GFP) antibody (ABIN1742293) (GFP anticorps)

2 images

(1 reference)

N° du produit ABIN1742293

Fiche de données

Antigène Voir toutes GFP Anticorps

GFP (Green Fluorescent Protein (GFP))
Reactivité
178

19

15

15

12

7

5

4

2

1

1

1

1
Aequorea victoria
Hôte
74

73

35

11

9

6

5

1
Lapin
Anticorps secondaires appropriés
Clonalité
121

88
Polyclonal
Conjugué
105

14

12

9

6

5

4

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Cet anticorp GFP est non-conjugé
Application
173

87

56

42

33

32

31

21

16

14

14

14

12

6

4

4

3

3

2

1

1

1

1

1

1
Western Blotting (WB), Immunocytochemistry (ICC)

Réactivité croisée (Details)

Cross reacts with all GFP derivatives tested so far (e.g. EYFP, ECFP, EGFP).

Purification

Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Immunogène

Strep-Tag® fusion protein, full-length GFP.

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anti-Green Fluorescent Protein (GFP) (AA 246) antibody (FITC)
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Indications d'application

WB: 1 : 1000 up to 1 : 20000 (AP staining)
IP: not tested yet
ICC: 1 : 100 up to 1 : 1000
IHC-P: not tested yet

Commentaires

ABIN1742297 Suitable for internalization studies when GFP is fused to an epitope residing in the lumen of an acidic compartment.

Restrictions

For Research Use only
Format

Lyophilized

Reconstitution

For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer

PBS

Conseil sur la manipulation

Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Stock

-20 °C

Stockage commentaire

Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
Rotmann, Strand, Martiné, Closs: "Protein kinase C activation promotes the internalization of the human cationic amino acid transporter hCAT-1. A new regulatory mechanism for hCAT-1 activity." dans: The Journal of biological chemistry, Vol. 279, Issue 52, pp. 54185-92, (2004) (PubMed).
Antigène

GFP (Green Fluorescent Protein (GFP))

Autre désignation

GFP (GFP Produits)

Synonymes

anticorps green fluorescent protein, anticorps gfp

Classe de substances

Viral Protein