CACNA1B anticorps (AA 1921-2222)

N° du produit ABIN1742328

Détail du produit anti-CACNA1B anticorps

Antigène: CACNA1B (Calcium Channel, Voltage-Dependent, N Type, alpha 1B Subunit (CACNA1B))

Épitope: AA 1921-2222

Reactivité: Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CACNA1B est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Specificité: Specific for Ca2+ channel alpha-1E.

Purification: Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Immunogène: Recombinant protein (aa 1921-2222 of rat Ca2+-channel alpha-1E subunit).

Information d'application

Indications d'application: WB: 1 : 500 up to 1 : 1000
IP: not tested yet
ICC: not tested yet
IHC: 1 : 500

Commentaires:

WB: Due to its large size, this antibody requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels of anamed or NuPage TRIS-acetate gels from Invitrogen. WB: This protein tends to aggregate after boiling, making it necessary to run SDS-PAGE with non-boiled samples. ECL detection is recommended: IHC: This antibody requires a mild fixation protocol (1 % PFA in sodium acetate buffer, pH 6).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Conseil sur la manipulation: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Stock: -20 °C

Stockage commentaire: Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

Détails sur CACNA1B

Antigène: CACNA1B (Calcium Channel, Voltage-Dependent, N Type, alpha 1B Subunit (CACNA1B))

Autre désignation: Ca2+ Channel (CACNA1B Produits)

Synonymes: anticorps BIII, anticorps CACNL1A5, anticorps CACNN, anticorps Cav2.2, anticorps AW050276, anticorps AW060892, anticorps AW822256, anticorps Cchn1a, anticorps alpha(1B), anticorps calcium voltage-gated channel subunit alpha1 B, anticorps calcium channel, voltage-dependent, N type, alpha 1B subunit, anticorps CACNA1B, anticorps Cacna1b

Sujet: Synonyms: Cav2.3