Abeta-pE3 anticorps

N° du produit ABIN1742433

Détail du produit

Antigène: Abeta-pE3

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Specificité: Specific for Abeta-pE3.

Purification: purified IgG. Azide was added before lyophilization.

Immunogène: Synthetic peptide pyr-EFRHD coupled to key-hole limpet hemocyanin via an added C-terminal cysteine residue. Epitope Minimal epitope: pyr-EFR.

Clone: 1-57

Isotype: IgG2b

Information d'application

Indications d'application: WB: 1 : 1000
ICC: not tested yet
IHC: 1 : 100

Commentaires:

WB: Detects purified Abeta pE3. Complex samples like brain extracts still have to be tested.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS, 0.02% sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.

Stock: -20 °C

Stockage commentaire: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

Référence

Antonios, Saiepour, Bouter, Richard, Paetau, Verkkoniemi-Ahola, Lannfelt, Ingelsson, Kovacs, Pillot, Wirths, Bayer: "N-truncated Abeta starting with position four: early intraneuronal accumulation and rescue of toxicity using NT4X-167, a novel monoclonal antibody." dans: Acta neuropathologica communications, Vol. 1, Issue 1, pp. 56, (2013) (PubMed).

Wittnam, Portelius, Zetterberg, Gustavsson, Schilling, Koch, Demuth, Blennow, Wirths, Bayer: "Pyroglutamate amyloid β (Aβ) aggravates behavioral deficits in transgenic amyloid mouse model for Alzheimer disease." dans: The Journal of biological chemistry, Vol. 287, Issue 11, pp. 8154-62, (2012) (PubMed).

Détail du antigène

Antigène: Abeta-pE3

Sujet: Synonyms: pyro-Glu Abeta, pE-Abeta, pGlu-Abeta, Abeta(3(pE)-40/42)