GABRA1 anticorps (AA 28-43)
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- Antigène Voir toutes GABRA1 Anticorps
- GABRA1 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 1 (GABRA1))
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Épitope
- AA 28-43
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Reactivité
- Rat, Souris
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp GABRA1 est non-conjugé
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
- Specificité
- Specific for GABA-A receptor alpha1.
- Purification
- purified IgM. Azide was added before lyophilization.
- Immunogène
- Synthetic peptide (aa 28-43 of rat GABA-A receptor alpha1 precursor protein) coupled to key-hole limpet hemocyanin via an added C-terminal cysteine residue.
- Clone
- 53D3
- Isotype
- IgM
- Top Product
- Discover our top product GABRA1 Anticorps primaire
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- Indications d'application
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WB: 1 : 1000 up to 1 : 2000 (AP staining)
IP: not tested yet
ICC: 1 : 500
IHC: 1 : 500 - Commentaires
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ICC: This antibody is PFA fixation sensitive, use only mild fixation (2 % PFA). Best results are obtained by application of antibodies on living cells. After washing cells with bound antibodies, they can be fixed and visualized with secondary reagents.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
- Buffer
- PBS, 0.02% sodium azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.
- Stock
- -20 °C
- Stockage commentaire
- Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
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-
How to Make an Active Zone: Unexpected Universal Functional Redundancy between RIMs and RIM-BPs." dans: Neuron, Vol. 91, Issue 4, pp. 792-807, (2016) (PubMed).
: "
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How to Make an Active Zone: Unexpected Universal Functional Redundancy between RIMs and RIM-BPs." dans: Neuron, Vol. 91, Issue 4, pp. 792-807, (2016) (PubMed).
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- Antigène
- GABRA1 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 1 (GABRA1))
- Autre désignation
- GABA-A Receptor Alpha1 (GABRA1 Produits)
- Synonymes
- anticorps ECA4, anticorps EJM, anticorps EJM5, anticorps zgc:153566, anticorps Gabra-1, anticorps gamma-aminobutyric acid type A receptor alpha1 subunit, anticorps gamma-aminobutyric acid (GABA) A receptor, alpha 1, anticorps gamma-aminobutyric acid (GABA) A receptor, subunit alpha 1, anticorps gamma-aminobutyric acid receptor subunit alpha-1, anticorps GABRA1, anticorps Gabra1, anticorps gabra1, anticorps LOC100653509
- Sujet
- Synonyms: GABA(A) R alpha1
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