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GABRA1 anticorps (AA 28-43)

GABRA1 Reactivité: Rat, Souris WB, IHC, ICC Hôte: Souris Monoclonal 53D3 unconjugated
N° du produit ABIN1742455
  • Antigène Voir toutes GABRA1 Anticorps
    GABRA1 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 1 (GABRA1))
    Épitope
    • 21
    • 20
    • 20
    • 20
    • 15
    • 15
    • 15
    • 14
    • 11
    • 11
    • 9
    • 5
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 28-43
    Reactivité
    • 130
    • 106
    • 86
    • 14
    • 8
    • 6
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Rat, Souris
    Hôte
    • 101
    • 73
    • 2
    Souris
    Clonalité
    • 101
    • 75
    Monoclonal
    Conjugué
    • 53
    • 12
    • 11
    • 11
    • 10
    • 9
    • 9
    • 9
    • 9
    • 9
    • 6
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    Cet anticorp GABRA1 est non-conjugé
    Application
    • 166
    • 80
    • 75
    • 70
    • 33
    • 28
    • 26
    • 26
    • 17
    • 12
    • 8
    • 4
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
    Specificité
    Specific for GABA-A receptor alpha1.
    Purification
    purified IgM. Azide was added before lyophilization.
    Immunogène
    Synthetic peptide (aa 28-43 of rat GABA-A receptor alpha1 precursor protein) coupled to key-hole limpet hemocyanin via an added C-terminal cysteine residue.
    Clone
    53D3
    Isotype
    IgM
    Top Product
    Discover our top product GABRA1 Anticorps primaire
  • Indications d'application
    WB: 1 : 1000 up to 1 : 2000 (AP staining)
    IP: not tested yet
    ICC: 1 : 500
    IHC: 1 : 500
    Commentaires

    ICC: This antibody is PFA fixation sensitive, use only mild fixation (2 % PFA). Best results are obtained by application of antibodies on living cells. After washing cells with bound antibodies, they can be fixed and visualized with secondary reagents.

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
    Buffer
    PBS, 0.02% sodium azide
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.
    Stock
    -20 °C
    Stockage commentaire
    Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
  • Acuna, Liu, Südhof: "How to Make an Active Zone: Unexpected Universal Functional Redundancy between RIMs and RIM-BPs." dans: Neuron, Vol. 91, Issue 4, pp. 792-807, (2016) (PubMed).

  • Antigène
    GABRA1 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 1 (GABRA1))
    Autre désignation
    GABA-A Receptor Alpha1 (GABRA1 Produits)
    Synonymes
    anticorps ECA4, anticorps EJM, anticorps EJM5, anticorps zgc:153566, anticorps Gabra-1, anticorps gamma-aminobutyric acid type A receptor alpha1 subunit, anticorps gamma-aminobutyric acid (GABA) A receptor, alpha 1, anticorps gamma-aminobutyric acid (GABA) A receptor, subunit alpha 1, anticorps gamma-aminobutyric acid receptor subunit alpha-1, anticorps GABRA1, anticorps Gabra1, anticorps gabra1, anticorps LOC100653509
    Sujet
    Synonyms: GABA(A) R alpha1
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