GABRB1 anticorps (AA 342-430)

N° du produit ABIN1742466

Détail du produit anti-GABRB1 anticorps

Antigène: GABRB1 (gamma-aminobutyric Acid (GABA) A Receptor, Subunit beta 1 (GABRB1))

Épitope: AA 342-430

Reactivité: Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GABRB1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Specificité: Specific for GABA-A receptor beta1.

Purification: Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Immunogène: Recombinant protein (aa 342-430 in mouse) of the unprocessed precursor forced into a loop-shaped structure.

Information d'application

Indications d'application: WB: 1 : 1000 (AP staining)
IP: not tested yet
ICC: 1 : 500
IHC: 1 : 500

Commentaires:

WB: This protein aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples. IHC: This antibody requires antigen retrieval with pepsin according to: Lorincz A & Nusser Z (2008).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Conseil sur la manipulation: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Stock: -20 °C

Stockage commentaire: Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

Références pour anticorps anti-GABRB1 (ABIN1742466)

Kerti-Szigeti, Nusser: "Similar GABAA receptor subunit composition in somatic and axon initial segment synapses of hippocampal pyramidal cells." dans: eLife, Vol. 5, (2016) (PubMed).

Détails sur GABRB1

Antigène: GABRB1 (gamma-aminobutyric Acid (GABA) A Receptor, Subunit beta 1 (GABRB1))

Autre désignation: GABA-A Receptor Beta1 (GABRB1 Produits)

Synonymes: anticorps AW061132, anticorps B230208N19Rik, anticorps Gabrb-1, anticorps GARB1, anticorps si:dkey-20i10.4, anticorps gamma-aminobutyric acid type A receptor beta1 subunit, anticorps gamma-aminobutyric acid (GABA) A receptor, subunit beta 1, anticorps gamma-aminobutyric acid type A receptor beta 1 subunit, anticorps gamma-aminobutyric acid (GABA) A receptor, beta 1, anticorps GABRB1, anticorps Gabrb1, anticorps gabrb1

Sujet: Synonyms: GABA(A) R beta1