BMP4 anticorps

N° du produit ABIN181146

Cet anticorps anti-BMP4 est un anticorps Souris Monoclonal détectant BMP4 dans WB, IF, EIA et Func. Adapté pour Humain.

Aperçu rapide pour BMP4 anticorps (ABIN181146)

Antigène: BMP4 (Bone Morphogenetic Protein 4 (BMP4))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp BMP4 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Functional Studies (Func)

Détail du produit anti-BMP4 anticorps

Specificité: This antibody detects BMP4.

Attributs du produit: Synonyms: BMP2B, BMP-2B, BMP-4, DVR4, Bone morphogenetic protein 4

Purification: Affinity Chromatography on Protein G

Immunogène: Highly pure (>98%) recombinant Human BMP-4

Isotype: IgG2b

Information d'application

Indications d'application: ELISA: In a sandwich ELISA (assuming 100μL/well), a concentration of 4.0-8.0 μg/mL of thisantibody will detect at least 2000 pg/mL of recombinant Human BMP-4 when used inconjunction with compatible secondary reagents. Western Blot: To detect Human BMP-4 by Western Blot analysis this antibody can be usedat a concentration of 0.25-0.50 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant Human BMP-4 is 2.0-4.0 ng/lane, undernon-reducing conditions. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhBMP-4 (5.0 ng/mL), a concentration of 0.4-0.6 μg/mL of this antibody is required.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in sterile water to a concentration of 1.0 mg/mL.

Buffer: PBS without preservatives

Agent conservateur: Without preservative

Stock: 4 °C/-20 °C

Stockage commentaire: The lyophilized antibody is stable at room temperature for one month or at -20 °C forlonger. Following reconstitution it is stable at 2-8 °C for six weeks. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur BMP4

Antigène: BMP4 (Bone Morphogenetic Protein 4 (BMP4))

Autre désignation: BMP4

Sujet: BMPs (bone morphogenetic proteins) belong to the TGF beta superfamily of structurally related signaling proteins. Members of this superfamily are widely represented throughout the animal kingdom and have been implicated in a variety of developmental processes. Proteins of the TGF beta superfamily are disulfide linked dimers composed of two 12-15 kDa polypeptide chains. As implied by their name, BMPs initiate, promote and regulate bone development, growth, remodeling and repair. Smad1 translocation to the nucleus is observed after the addition of BMP4 (also designated BMP2B), suggesting that BMP4 may play a role in activation of the Smad pathway. BMP is secreted into the extracellular matrix.Synonyms: BMP-2B, BMP-4, BMP2B, Bone morphogenetic protein 4, DVR4

ID gène: 652

UniProt: P12644

Pathways: Steroid Hormone Mediated Signaling Pathway, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development