VEGFA anticorps

N° du produit ABIN181548

Détail du produit anti-VEGFA anticorps

Antigène: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp VEGFA est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Specificité: This antibody detects VEGF.

Purification: Immunoaffinity Chromatography

Immunogène: Highly pure (> 98 %) recombinant human VEGF.

Information d'application

Indications d'application: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofHuman VEGF (10 ng/mL), a concentration of 0.05-0.1 μg/mL of this antibody is required. Sandwich ELISA: To detect Human VEGF by Sandwich ELISA (using 100 μL/well antibodysolution) a concentration of 0.5-2.0 μg/mL is required. In conjunction with BiotinylatedAnti-Human VEGF (ABIN181546 or ABIN181545) as a Detection antibody, it allows thedetection of at least 0.2-0.4 ng/well of recombinant Human VEGF. Western Blot: To detect Human VEGF by Western Blot analysis this antibody can be used ata concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant Human VEGF is 1.5-3.0 ng/lane, under either reducingor non-reducing conditions. Immunohistochemistry: 5.0-15.0 μg/mL with 30 minutes at RT or an overnight incubation at4 °C. This antibody stained Formalin-Fixed, Paraffin-Embedded sections of Human breastinvasive ductal carcinoma. An HRP-labeled polymer detection system was used with a DABchromogen. This antibody was also tested in an HRP-Biotin labeling detection system. Heat induced antigen retrieval with pH 6.0 Sodium Citrate buffer is recommended.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2-8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Date de péremption: 6 months

Détails sur VEGFA

Antigène: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Autre désignation: VEGF-A (VEGFA Produits)

Synonymes: anticorps MVCD1, anticorps VEGF, anticorps VPF, anticorps Vegf, anticorps Vegf120, anticorps Vegf164, anticorps Vegf188, anticorps Vpf, anticorps vegf, anticorps vegfa, anticorps wu:fj82c06, anticorps VEGF-A, anticorps VEGF164, anticorps eVEGF120, anticorps eVEGF164, anticorps vegf-a, anticorps vpf, anticorps vefg, anticorps si:dkey-14d8.3, anticorps wu:fd42e02, anticorps vascular endothelial growth factor A, anticorps vascular endothelial growth factor Aa, anticorps vascular endothelial growth factor A L homeolog, anticorps vascular endothelial growth factor Ab, anticorps VEGFA, anticorps Vegfa, anticorps vegfaa, anticorps vegfa.L, anticorps vegfa, anticorps vegfab

Sujet: Synonyms: VEGF, VEGFA, VPF, Vascular endothelial growth factor A, Vascular permeability factor

ID gène: 7422

NCBI Accession: NP_001020537

UniProt: P15692

Pathways: Signalisation RTK, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling