RANKL anticorps

N° du produit ABIN181556

Cet anticorps Chèvre Polyclonal détecte spécifiquement RANKL dans WB et EIA. Il présente une réactivité envers Humain.

Aperçu rapide pour RANKL anticorps (ABIN181556)

Antigène: RANKL (TNFSF11) (Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp RANKL est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Détail du produit anti-RANKL anticorps

Specificité: This antibody detects RANKL.

Purification: Immunoaffinity purified

Immunogène: Highly pure (> 98 %) recombinant human sRANKL

Isotype: IgG

Information d'application

Indications d'application: ELISA: Indirect: To detect hsRANKL by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL of this antibody is required. In conjunction withcompatible secondary reagents, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hsRANKL. Sandwich: To detect hsRANKL by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL of this antibody is required. In conjunction withBiotinylated Anti-Human sRANKL as a detection antibody, it allows the detection of at least0.2 - 0.4 ng/well of recombinant hsRANKL. Western Blot: To detect hsRANKL by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hsRANKL is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.

Restrictions: For Research Use only

Stockage

Reconstitution: Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1 - 1.0 mg/mL.

Buffer: PBS, pH 7.2

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Date de péremption: 6 months

Détails sur RANKL

Antigène: RANKL (TNFSF11) (Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11))

Autre désignation: CD254 / RANKL

Sujet: RANKL is a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. There are three isoforms of RANKL. Human RANKL is a soluble 20 kDa polypeptide, comprising the TNF homologous region of RANKL (176 amino acid residues). This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor associated factor (TRAF) 6, which indicated that this protein may have a role in the regulation of cell apoptosis. RANKL deficient mice show severe osteoporesis and complete absence of osteoclasts as a result of lack of osteogenesis.Synonyms: ODF, OPGL, Osteoclast differentiation factor, Osteoprotegerin ligand, RANK Ligand, RANKL, Receptor activator of nuclear factor kappa B ligand, TNF-related activation-induced cytokine, TNFSF11, TRANCE, Tumor necrosis factor ligand superfamily member 11

Poids moléculaire: Approx. 150 kDa

ID gène: 8600

NCBI Accession: NP_003692

UniProt: O14788

Pathways: Signalisation NF-kappaB