GRB2 anticorps (C-Term)

N° du produit ABIN184670

L’anticorps Chèvre Polyclonal anti-GRB2 a été validé pour WB, ELISA et IHC. Il convient pour détecter GRB2 dans des échantillons de Humain, Souris, Rat et Porc.

Aperçu rapide pour GRB2 anticorps (C-Term) (ABIN184670)

Antigène: GRB2 (Growth Factor Receptor-Bound Protein 2 (GRB2))

Reactivité: Humain, Souris, Rat, Porc

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp GRB2 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Classe de qualité: Verified

Détail du produit anti-GRB2 anticorps

Épitope: C-Term

Fonction: GRB2

Séquence: PRNYVTPVNR NV

Specificité: This antibody is expected to recognize both reported isoforms (NP_002077.1and NP_987102.1).

Réactivité croisée: Boeuf (Vache), Chien, Humain, Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-PRNYVTPVNRNV, from the C Terminus of the protein sequence according to NP_002077.1, NP_987102.1.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: In paraffin embedded Human Lymph Node shows nuclear staining in some cells inside the germinal centre. Recommended concentration: 1-2 μg/mL.

Western Blot: Approx 26 kDa band observed in lysates of cell line MOLT4, of Human Thymus and of Human, Mouse, Rat, Pig Brain and Spleen lysates (calculated MW of 25.2 kDa according to Human NP_002077.1 and according to Rat NP_110473.2). Recommended for use

Peptide ELISA: antibody detection limit dilution 1:32000.

Commentaires:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur GRB2

Antigène: GRB2 (Growth Factor Receptor-Bound Protein 2 (GRB2))

Autre désignation: GRB2

Sujet: GRB2, growth factor receptor-bound protein 2, ASH, ASH protein, EGFRBP-GRB2, Grb3-3, MST084, MSTP084, HT027, abundant SRC homology, epidermal growth factor receptor-binding protein GRB2, growth factor receptor-bound protein 3, NCKAP2, OTTHUMP00000166096,

ID gène: 2885, 14784, 81504

NCBI Accession: NP_002077, NP_987102

Pathways: Signalisation RTK, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Hepatitis C, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, EGFR Downregulation