SIAH1 anticorps (N-Term)

N° du produit ABIN184675

Cet anticorps Chèvre Polyclonal détecte spécifiquement SIAH1 dans WB, ELISA et IF. Il présente une réactivité envers Humain et Rat et a été mentionné dans 1 publication.

Aperçu rapide pour SIAH1 anticorps (N-Term) (ABIN184675)

Antigène: SIAH1 (E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1))

Reactivité: Humain, Rat

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp SIAH1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Classe de qualité: Verified

Détail du produit anti-SIAH1 anticorps

Épitope: N-Term

Fonction: SIAH1

Séquence: SRQTATALPT GTSKC

Specificité: This product is expected to recognize both reported isoforms (NP_003022.3, NP_001006611.1)

Réactivité croisée: Boeuf (Vache), Chien, Humain, Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence SRQTATALPTGTSKC, from the N Terminus of the protein sequence according to NP_003022.3, NP_001006611.1.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Approx. 37 kDa band observed in Human Liver lysates and approx. 30+37 kDa bands in Rat Liver lysates (calculated MW of 34.6 kDa according to Human NP_001006611.1 and 31.1 kDa according to Rat NP_543181.2 and 38.2 kDa according to Rat XP_03895336

Peptide ELISA: antibody detection limit dilution 1:128000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the nucleus and cytoplasm of U2OS and HepG2 cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Références pour anticorps anti-SIAH1 (ABIN184675)

Sroka, Voigt, Deeg, Reed, Schulz, Bhr, Kermer: "BAG1 modulates huntingtin toxicity, aggregation, degradation, and subcellular distribution." dans: Journal of neurochemistry, Vol. 111, Issue 3, pp. 801-7, (2009) (PubMed).

Détails sur SIAH1

Antigène: SIAH1 (E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1))

Autre désignation: SIAH1

Sujet: SIAH1, seven in absentia homolog 1 (Drosophila), Siah-1, hSIAH1, HUMSIAH, Siah-1a, FLJ08065, seven in absentia homolog 1, sonic hedgehog homolog

Poids moléculaire: 31.1kDa

ID gène: 6477, 20437, 140941

NCBI Accession: NP_003022, NP_001006611