BAF53A and BAF53B (C-Term) anticorps

N° du produit ABIN185217

Détail du produit

Antigène: BAF53A and BAF53B

Épitope: C-Term

Reactivité: Humain, Souris, Rat

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF)

Fonction: BAF53A and BAF53B

Séquence: YEEGGKQCVE RKCP

Specificité: This antibody is expected to recognise BAF53A isoforms 1 and 2 and BAF53B, which are almost identical

Réactivité croisée: Boeuf (Vache), Chien, Humain, Souris, Porc, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Classe de qualité: Verified

Immunogène: Peptide with sequence YEEGGKQCVERKCP, from the C Terminus of the protein sequence according to NP_004292.1, NP_817126.1, NP_057272.1.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Approx 48 kDa band observed in Rat Skeletal Muscle lysates and approx. 50 kDa in nuclear lysates of cell line NIH3T3 and in preliminary testing of Human Colorectal cancer and Mouse Skeletal Muscle lysate.(predicted MW of 47.4 kDa (Actl6a) acco

Peptide ELISA: antibody detection limit dilution 1:16000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the nuclei of A431 and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10u

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détail du antigène

Antigène: BAF53A and BAF53B

Autre désignation: ACTL6A / ACTL6B

Synonymes: anticorps ACTL6, anticorps BAF53B, anticorps Actl6, anticorps ArpNa, anticorps Baf53b, anticorps actin like 6B, anticorps actin-like 6B, anticorps ACTL6B, anticorps Actl6b

Sujet: BAF53A, BAF53B, ACTL6, BAF53a, BAF53b, actin-like 6A, actin-like 6B, hArpN alpha, actin-related protein, ACTL6A, ACTL6B, 53 kDa BRG1-associated factor B, ARPN-BETA, MGC5382 Arp4, INO80K, BAF complex 53 kDa subunit, BAF53, BRG1-associated factor, INO80 com

ID gène: 51412, 86

NCBI Accession: NP_004292, NP_817126, NP_057272