IRAK4 anticorps (N-Term)

N° du produit ABIN185338

Cet anticorps Chèvre Polyclonal détecte spécifiquement IRAK4 dans WB, ELISA, IHC, FACS et IF. Il présente une réactivité envers Humain.

Aperçu rapide pour IRAK4 anticorps (N-Term) (ABIN185338)

Antigène: IRAK4 (Interleukin-1 Receptor-Associated Kinase 4 (IRAK4))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp IRAK4 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Classe de qualité: Verified

Détail du produit anti-IRAK4 anticorps

Épitope: N-Term

Fonction: IRAK4 (N Terminus)

Séquence: NKPITPSTYV RC

Réactivité croisée: Humain, Souris

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: NKPITPSTYVRC

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: Paraffin embedded Human Heart and Human Liver. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 50 kDa band observed in lysates of cell line HeLa and approx 55 kDa in lysates of cell line Jurkat (calculated MW of 51.5 kDa according to NP_057207.1). This molecular weight is routinely observed by other sources. Recommended concentra

Peptide ELISA: antibody detection limit dilution 1:32000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and microtubules of HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concent

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur IRAK4

Antigène: IRAK4 (Interleukin-1 Receptor-Associated Kinase 4 (IRAK4))

Autre désignation: IRAK4

Sujet: IRAK4, REN64, NY-REN-64, interleukin-1 receptor-associated kinase 4, IRAK-4 mutated form 1, interleukin-1 receptor associated kinase 4, interleukin-1 receptor associated kinase 4 mutant form 1, IPD1

ID gène: 51135, 266632

NCBI Accession: NP_057207, NP_001107654

Pathways: Signalisation NF-kappaB, Signalisation TLR, Activation of Innate immune Response, Toll-Like Receptors Cascades