MYD88 anticorps (Internal Region)

N° du produit ABIN185362

Cet anticorps Chèvre Polyclonal détecte spécifiquement MYD88 dans WB, IHC, ELISA, IF et FACS. Il présente une réactivité envers Humain.

Aperçu rapide pour MYD88 anticorps (Internal Region) (ABIN185362)

Antigène: MYD88 (Myeloid Differentiation Primary Response Gene (88) (MYD88))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp MYD88 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Classe de qualité: Verified

Détail du produit anti-MYD88 anticorps

Épitope: Internal Region

Fonction: MYD88

Séquence: IKYKAMKKEF P

Specificité: This antibody is expected to recognize reported isoforms 1, 2, 3 and 9.

Réactivité croisée: Chien, Humain, Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-IKYKAMKKEFP, from the internal region of the protein sequence according to NP_002459.1.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: Paraffin embedded Human Tonsil. Recommended concentration: 4-6 μg/mL.

Western Blot: Approx 28 kDa band observed in Human Spleen lysates (calculated MW of 28.3 kDa according to NP_001166039.2). Recommended concentration: 0.3-0.5 μg/mL. Primary incubation1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:128000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in U2OS and Jurkat cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur MYD88

Antigène: MYD88 (Myeloid Differentiation Primary Response Gene (88) (MYD88))

Autre désignation: MYD88

Sujet: MYD88, myeloid differentiation primary response gene (88), MYD88D, myeloid differentiation primary response gene 88

Poids moléculaire: 33.2kDa

ID gène: 4615, 17874, 301059

NCBI Accession: NP_001166038, NP_002459, NP_001166039

Pathways: Signalisation NF-kappaB, Signalisation TLR, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades