Patched 1 anticorps (C-Term)

N° du produit ABIN185521

Cet anticorps Chèvre Polyclonal détecte spécifiquement Patched 1 dans ELISA, IHC et IF. Il présente une réactivité envers Humain et Souris et a été mentionné dans 2+ publications.

Aperçu rapide pour Patched 1 anticorps (C-Term) (ABIN185521)

Antigène: Patched 1 (PTCH1)

Reactivité: Humain, Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Patched 1 est non-conjugé

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Classe de qualité: Verified

Détail du produit anti-Patched 1 anticorps

Épitope: C-Term

Fonction: PTCH (C Terminus)

Séquence: ELQDVECEER PR

Specificité: This antibody is expected to recognise all reported isoforms. Variants (NP_001077073.1, NP_001077074.1, NP_001077075.1, NP_001077076.1) encode the same isoform.

Réactivité croisée: Humain, Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence ELQDVECEERPR, from the C Terminus of the protein sequence according to NP_000255.1, NP_001077072.1, NP_001077071.1, NP_001077073.1, NP_001077074.1, NP_001077075.1, NP_001077076.1.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: Paraffin embedded Human Brain (Coretx). Recommended concentration: 8 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the Golgi of HeLa and NIH3T3 cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Références pour anticorps anti-Patched 1 (ABIN185521)

Abiria, Williams, Munden, Grover, Wallace, Lundberg, Valadez, Cooper: "Expression of Hedgehog ligand and signal transduction components in mutually distinct isocitrate dehydrogenase mutant glioma cells supports a role for paracrine signaling." dans: Journal of neuro-oncology, Vol. 119, Issue 2, pp. 243-51, (2014) (PubMed).

Gerardo Valadez, Grover, Carter, Calcutt, Abiria, Lundberg, Williams, Cooper: "Identification of Hedgehog pathway responsive glioblastomas by isocitrate dehydrogenase mutation." dans: Cancer letters, Vol. 328, Issue 2, pp. 297-306, (2012) (PubMed).

Détails sur Patched 1

Antigène: Patched 1 (PTCH1)

Autre désignation: PTCH1

Sujet: PTCH1, PTCH, patched homolog (Drosophila), HGNC:9585, BCNS, HPE7, NBCCS, PTC, PTC1, patched, patched (Drosophila) homolog, patched homolog 1 (Drosophila), RP11-435O5.3, FLJ26746, FLJ42602, PTCH11, OTTHUMP00000021709, OTTHUMP00000021710, PTCH protein +12b,

ID gène: 5727

NCBI Accession: NP_000255, NP_001077072, NP_001077071, NP_001077073, NP_001077074, NP_001077075, NP_001077076

Pathways: Signalisation Hedgehog, Carbohydrate Homeostasis, Tube Formation