PDE4D anticorps (C-Term)

N° du produit ABIN185530

Cet anticorps Chèvre Polyclonal détecte spécifiquement PDE4D dans WB, ELISA, IHC et EIA. Il présente une réactivité envers Humain et a été mentionné dans 1 publication.

Aperçu rapide pour PDE4D anticorps (C-Term) (ABIN185530)

Antigène: PDE4D (phosphodiesterase 4D, cAMP-Specific (PDE4D))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp PDE4D est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Enzyme Immunoassay (EIA)

Classe de qualité: Verified

Détail du produit anti-PDE4D anticorps

Épitope: C-Term

Fonction: PDE4D

Séquence: QPEACVIDDR SPDT

Specificité: This antiobody is expected to recognize all reported isoforms.

Réactivité croisée: Humain

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence QPEACVIDDRSPDT, from the C Terminus of the protein sequence according to NP_001184151.1, NP_001184150.1, NP_006194.2, NP_001098101.1, NP_001184152.1, NP_001159371.1.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: Paraffin embedded Human Skeletal Muscle and Heart. Recommended concentration: 5 μg/mL.

Western Blot: Approx 70+80+90 kDa bands observed in lysates of cell line A549. In transfected COS1 transiently expressing PDE4D1 and PDE4D3 bands of approx. 68 kDa and 95 kDa are observed. These bands are not observed in the untransfected COS1. Calculated

Peptide ELISA: antibody detection limit dilution 1:128000.

Commentaires:

ELISA: This antibody has been successfully used in ELISA as described in the following paper: Bazhin et al, Mol Immunol. 2010 Jan,47(4):678-84., PMID: 19939455.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Références pour anticorps anti-PDE4D (ABIN185530)

Bazhin, Kahnert, Kimpfler, Schadendorf, Umansky: "Distinct metabolism of cyclic adenosine monophosphate in regulatory and helper CD4+ T cells." dans: Molecular immunology, Vol. 47, Issue 4, pp. 678-84, (2010) (PubMed).

Détails sur PDE4D

Antigène: PDE4D (phosphodiesterase 4D, cAMP-Specific (PDE4D))

Autre désignation: PDE4D

Sujet: PDE4D, phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila), HGNC:8783, DPDE3, HSPDE4D, PDE4DN2, STRK1, cAMP-specific phosphodiesterase 4D, cAMP-specific phosphodiesterase PDE4D6, dunce-like phosphodiesterase E3, phosphodie

ID gène: 5144

NCBI Accession: NP_001184151, NP_001184150, NP_006194, NP_001098101, NP_001184152, NP_001159371

Pathways: Cellular Response to Molecule of Bacterial Origin, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling