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CD14 anticorps (Internal Region)

Name: CD14 anticorps (Internal Region)
Brand: antibodies-online
SKU: ABIN185563
Price: 809.48 EUR
Availability: InStock

Verified CD14 Reactivité: Humain FACS, WB, ELISA Hôte: Chèvre Polyclonal unconjugated

(ABIN185563) Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.

FACS

(ABIN185563) (2 μg/mL) staining of Human Lymph node (A) + peptide (B) and Tonsil (C) + peptide (D) lysate (35 μg protein in RIPA buffer) Detected by chemiluminescence.

ABIN185563 (1µg/ml) staining of Human Lymph Node lysate (35µg protein in RIPA buffer).

ABIN185563 (1µg/ml) staining of PBM lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide.

ABIN185563 (4µg/ml) staining of paraffin embedded Human Spleen.

IHC

5 images

N° du produit ABIN185563

Fiche de données

Antigène Voir toutes CD14 Anticorps

CD14
Épitope
16

9

7

6

5

4

4

3

3

3

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

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1
Internal Region
Reactivité
267

55

32

30

29

28

25

24

24

18

17

14

10

9

8

8

8

5

2

2

2

1

1

1
Humain
Hôte
225

63

25

2

1
Chèvre
Anticorps secondaires appropriés
Clonalité
253

63
Polyclonal
Conjugué
127

30

24

18

15

8

6

6

5

5

5

4

4

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Cet anticorp CD14 est non-conjugé
Application
223

102

63

59

53

49

22

22

21

14

14

13

7

6

4

4

3

3

3

3

3

3

2

2

2

2

1

1

1

1

1
Flow Cytometry (FACS), Western Blotting (WB), ELISA

Fonction

CD14

Séquence

KRVDADADPR QYAD

Réactivité croisée

Humain

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Classe de qualité

Verified

Immunogène

Peptide with sequence C-KRVDADADPRQYAD, from the internal region of the protein sequence according to NP_000582.1.

Isotype

IgG

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Indications d'application

Western Blot: Approx 55 kDa band observed in Human Lymph node and Tonsil lysates (calculated MW of 40.1 kDa according to NP_000582.1).The observed molecular weight corresponds to the glycosylated form, and was successfully blocked by incubation with the im
Peptide ELISA: antibody detection limit dilution 1:128000.

Commentaires

Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.

Restrictions

For Research Use only
Format

Liquid

Concentration

0.5 mg/mL

Buffer

Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur

Sodium azide

Précaution d'utilisation

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation

Minimize freezing and thawing.

Stock

-20 °C

Stockage commentaire

Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
Antigène

CD14

Autre désignation

CD14 (CD14 Produits)

Synonymes

anticorps CD14 molecule, anticorps CD14 antigen, anticorps CD14, anticorps Cd14

Sujet

CD14, CD14 antigen , HGNC:1628

ID gène

929

NCBI Accession

NP_000582

Pathways

Signalisation TLR, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades