Leptin Receptor anticorps (Internal Region)

N° du produit ABIN185639

L’anticorps Chèvre Polyclonal anti-Leptin Receptor a été validé pour WB, ELISA, IHC et FACS. Il convient pour détecter Leptin Receptor dans des échantillons de Humain, Souris et Rat.

Aperçu rapide pour Leptin Receptor anticorps (Internal Region) (ABIN185639)

Antigène: Leptin Receptor (LEPR)

Reactivité: Humain, Souris, Rat

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Leptin Receptor est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Classe de qualité: Verified

Détail du produit anti-Leptin Receptor anticorps

Épitope: Internal Region

Fonction: Leptin Receptor

Séquence: TQDDIEKHQS DAG

Specificité: This antibody is expected to recognise all three reported isoforms (NP_002294.2, NP_001003679.1, NP_001003680.1).

Réactivité croisée: Chien, Humain

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-TQDDIEKHQSDAG, from the internal region of the protein sequence according to NP_002294.2, NP_001003679.1, NP_001003680.1.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 4-6 μg/mL.

Western Blot: Approx 140-150 kDa band observed in Human Cerebellum, Mouse Foetal Brain and Rat Brain lysates (calculated MW of 132 kDa according to Human NP_002294.2 and 130.8 kDa according to Mouse NP_666258.2 and Rat NP_036728.1). This molecular weight is

Peptide ELISA: antibody detection limit dilution 1:128000.

Commentaires:

Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur Leptin Receptor

Antigène: Leptin Receptor (LEPR)

Autre désignation: LEPR

Sujet: LEPR , leptin receptor , CD295, OBR , OB receptor, LeprB

ID gène: 3953

NCBI Accession: NP_002294, NP_001003679, NP_001003680

Pathways: Signalistation JAK/STAT, AMPK Signaling, Feeding Behaviour