Patched 1 anticorps (Internal Region)

N° du produit ABIN185667

Cet anticorps anti-Patched 1 est un anticorps Chèvre Polyclonal détectant Patched 1 dans WB, ELISA et IF. Adapté pour Humain et Souris. Ce Primary Antibody a été cité dans 1 publication.

Aperçu rapide pour Patched 1 anticorps (Internal Region) (ABIN185667)

Antigène: Patched 1 (PTCH1)

Reactivité: Humain, Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Patched 1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Classe de qualité: Verified

Détail du produit anti-Patched 1 anticorps

Épitope: Internal Region

Fonction: PTCH (Internal)

Séquence: HPESRHHPPS NPRQQ

Specificité: This antibody is expected to recognise all four reported isoforms (NP_000255.2, NP_001077072.1, NP_001077071.1, NP_001077075.1). Reported variants represent identical protein (NP_001077075.1, NP_001077076.1, NP_001077073.1, NP_001077074.1).

Réactivité croisée: Boeuf (Vache), Chien, Humain, Porc

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-HPESRHHPPSNPRQQ, from the internal region of the protein sequence according to NP_000255.2, NP_001077072.1, NP_001077071.1, NP_001077075.1.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Approx 150 kDa band observed in Human Brain lysates (calculated MW of 153.9 kDa according to NP_001077071.1). Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:32000.

Commentaires:

Immunofluorescence :Expression of the protein seen in the Golgi/membrane of NIH3T3 cells. Recommended concentration: 5μg/ml.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Références pour anticorps anti-Patched 1 (ABIN185667)

Gerardo Valadez, Grover, Carter, Calcutt, Abiria, Lundberg, Williams, Cooper: "Identification of Hedgehog pathway responsive glioblastomas by isocitrate dehydrogenase mutation." dans: Cancer letters, Vol. 328, Issue 2, pp. 297-306, (2012) (PubMed).

Détails sur Patched 1

Antigène: Patched 1 (PTCH1)

Autre désignation: PTCH1

Sujet: PTCH, patched homolog (Drosophila), RP11-435O5.3, BCNS, FLJ42602, HPE7, NBCCS, PTC, PTC1, PTCH1 , PTCH protein +12b, PTCH protein +4', PTCH protein -10, patched, patched (Drosophila) homolog

ID gène: 5727

NCBI Accession: NP_000255, NP_001077072, NP_001077071, NP_001077075

Pathways: Signalisation Hedgehog, Carbohydrate Homeostasis, Tube Formation