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Bcl-2 anticorps (AA 2-208) (FITC)

L’anticorps Lapin Polyclonal anti-Bcl-2 a été validé pour WB, IHC, IF et ICC. Il convient pour détecter Bcl-2 dans des échantillons de Rat. Il y a 1 publication disponible.
N° du produit ABIN1858118

Aperçu rapide pour Bcl-2 anticorps (AA 2-208) (FITC) (ABIN1858118)

Antigène

Voir toutes Bcl-2 (BCL2) Anticorps
Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Reactivité

  • 434
  • 171
  • 167
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  • 9
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  • 2
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Rat

Hôte

  • 333
  • 142
  • 4
  • 2
  • 2
  • 1
  • 1
  • 1
Lapin

Clonalité

  • 318
  • 167
Polyclonal

Conjugué

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Cet anticorp Bcl-2 est conjugé à/à la FITC

Application

  • 366
  • 190
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  • 97
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Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
  • Épitope

    • 57
    • 39
    • 26
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    • 21
    • 16
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    • 1
    • 1
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    AA 2-208

    Fonction

    FITC-Linked Polyclonal Antibody to B-Cell Leukemia/Lymphoma 2 (Bcl2)

    Séquence

    MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- AQAGRTGYD NREIVMKYIH YKLSQRGYEW DTGDEDSAPL RAAPTPGIFS FQPESNRTPA VHRDTAARTS PLRPLVANAG PALSPVPPVV HLTLRRAGDD FSRRYRRDFA EMSSQLHLTP FTARGRFATV VEELFRDGVN WGRIVAFFEF GGVMCVESVN REMSPLVDNI ALWMTEYLNR HLHTWIQDNG GWDAFVELYG PSMRPLFD

    Specificité

    The antibody is a rabbit polyclonal antibody raised against Bcl2. It has been selected for its ability to recognize Bcl2 in immunohistochemical staining and western blotting.

    Purification

    Antigen-specific affinity chromatography followed by Protein A affinity chromatography

    Immunogène

    Recombinant Bcl2 expressed in E.coli.
    The antibody is a rabbit polyclonal antibody raised against Bcl2 conjugated to fitc.

    Isotype

    IgG
  • Indications d'application

    Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

    Commentaires

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    500 μg/mL

    Buffer

    PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

    Conseil sur la manipulation

    Avoid repeated freeze/thaw cycles

    Stock

    4 °C,-20 °C

    Stockage commentaire

    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

    Date de péremption

    12 months
  • Chen, Cao, Asare, Lv, Zhu, Li, Wei, Gao, Zhang, Mao, Gao, Fan: "Amelioration of cardiac dysfunction and ventricular remodeling after myocardial infarction by danhong injection are critically contributed by anti-TGF-β-mediated fibrosis and angiogenesis mechanisms." dans: Journal of ethnopharmacology, Vol. 194, pp. 559-570, (2016) (PubMed).

  • Antigène

    Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

    Autre désignation

    B-Cell Leukemia/Lymphoma 2

    Sujet

    B-Cell CLL/Lymphoma 2

    Pathways

    Signalisation MAPK, Signalisation PI3K-Akt, Apoptose, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling
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