FASL anticorps

N° du produit ABIN187288

Détail du produit anti-FASL anticorps

Antigène: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp FASL est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificité: Fas Ligand (FasL) is a type II membrane protein and a member of the tumor necrosis factor family. When interacting with the Fas receptor, the trimeric FasL induces apoptosis. It is responsible for the activation-induced apoptosis of peripheral T cells and functions as an effector molecule for cytotoxic T cells. FasL is expressed in activated lymphocytes, Sertoli cells, and some tumor cells. Species Reactivity: Mouse. Others not tested.

Immunogène: Synthetic peptide corresponding to aa 196-220 of the mouse Fas Ligand protein

Clone: A11

Isotype: IgM

Information d'application

Indications d'application: Flow cytometry Immunohistochemistry: Excellent for this application. Frozen Sections- Use 20 µg/mL, incubate 1 hr at room temp. Method: Cryostat sections (6-8 µm) were air dried, fixed in 4% paraformaldehyde in PBS and used immediately. Sections were incubated for 1 hr at RT with 20 µg/mL of A11 antibody in PBS, 0.2% BSA, 0.05% Tween 20. After rinsing in PBS, a FITC-conjugated anti-rat IgM was added for 30 min, slides washed again in PBS and visualized using a fluorescence microscope. Paraffin-Embedded Sections- Dewax in xylene (2 x 10 min), EtOH 100% (2 x 10 min), block endogenous peroxidase (0.6% H2O2 in Methanol 10 min), rehydrate through graded EtOH (100%, 95%, 70%), rinse in H2O, unmask epitopes by heating slides in citrate buffer (0.01 M, pH 6.0) 2 x 5 min in microwave oven (650-750W), cool down to RT 20 min, wash 1x in PBS, incubate with A11 20 µg/mL antibody, wash in PBS, incubate in anti-rat IgM HRP, wash in PBS, develop in appropriate substrate solution (DAB or AEC). The optimal dilution for a specific application should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 µL of 1 mg/mL monoclonal antibody in PBS with 0.02% sodium azide. Purity 95% by SDS-PAGE.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Détails sur FASL

Antigène: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Autre désignation: CD178 / Fas Ligand (FASL Produits)

Synonymes: anticorps ALPS1B, anticorps APT1LG1, anticorps APTL, anticorps CD178, anticorps CD95-L, anticorps CD95L, anticorps FASL, anticorps TNFSF6, anticorps fasl, anticorps Fas-L, anticorps Faslg, anticorps Tnfsf6, anticorps gld, anticorps Apt1Lg1, anticorps Fasl, anticorps FASLG, anticorps zgc:162027, anticorps Fas ligand, anticorps Fas ligand L homeolog, anticorps Fas ligand (TNF superfamily, member 6), anticorps FASLG, anticorps faslg.L, anticorps Fasl, anticorps Faslg, anticorps faslg

Pathways: Apoptose, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity