FASL anticorps

N° du produit ABIN187288

Cet anticorps Rat Monoclonal détecte spécifiquement FASL dans IHC (p) et IHC (fro). Il présente une réactivité avec des échantillons de Souris.

Aperçu rapide pour FASL anticorps (ABIN187288)

Antigène: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp FASL est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: A11

Détail du produit anti-FASL anticorps

Specificité: Fas Ligand (FasL) is a type II membrane protein and a member of the tumor necrosis factor family. When interacting with the Fas receptor, the trimeric FasL induces apoptosis. It is responsible for the activation-induced apoptosis of peripheral T cells and functions as an effector molecule for cytotoxic T cells. FasL is expressed in activated lymphocytes, Sertoli cells, and some tumor cells. Species Reactivity: Mouse. Others not tested.

Immunogène: Synthetic peptide corresponding to aa 196-220 of the mouse Fas Ligand protein

Isotype: IgM

Information d'application

Indications d'application: Flow cytometry Immunohistochemistry: Excellent for this application. Frozen Sections- Use 20 µg/mL, incubate 1 hr at room temp. Method: Cryostat sections (6-8 µm) were air dried, fixed in 4% paraformaldehyde in PBS and used immediately. Sections were incubated for 1 hr at RT with 20 µg/mL of A11 antibody in PBS, 0.2% BSA, 0.05% Tween 20. After rinsing in PBS, a FITC-conjugated anti-rat IgM was added for 30 min, slides washed again in PBS and visualized using a fluorescence microscope. Paraffin-Embedded Sections- Dewax in xylene (2 x 10 min), EtOH 100% (2 x 10 min), block endogenous peroxidase (0.6% H2O2 in Methanol 10 min), rehydrate through graded EtOH (100%, 95%, 70%), rinse in H2O, unmask epitopes by heating slides in citrate buffer (0.01 M, pH 6.0) 2 x 5 min in microwave oven (650-750W), cool down to RT 20 min, wash 1x in PBS, incubate with A11 20 µg/mL antibody, wash in PBS, incubate in anti-rat IgM HRP, wash in PBS, develop in appropriate substrate solution (DAB or AEC). The optimal dilution for a specific application should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 µL of 1 mg/mL monoclonal antibody in PBS with 0.02% sodium azide. Purity 95% by SDS-PAGE.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Détails sur FASL

Antigène: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Autre désignation: CD178 / Fas Ligand

Pathways: Apoptose, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity