FASL anticorps

N° du produit ABIN187289

L’anticorps Rat Monoclonal anti-FASL a été validé pour WB, FACS et IF. Il convient pour détecter FASL dans des échantillons de Souris.

Aperçu rapide pour FASL anticorps (ABIN187289)

Antigène: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp FASL est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: H11

Détail du produit anti-FASL anticorps

Specificité: Species Reactivity: Mouse. Others not tested.

Pureté: > 95 % by SDS-PAGE.

Immunogène: Synthetic peptide corresponding to aa 196-220 of the mouse Fas Ligand protein (CD95L, APO-1L, CD178).

Isotype: IgG2a

Information d'application

Indications d'application: Immunocytochemistry: Use anti-Fas Ligand, clone H11 unlabeled (Cat. No. MC-106), biotin-labeled (Cat. No. MC-126), or FITC-labeled (Cat. No. MC-136). Flow Cytometry: Use Anti-Fas Ligand, clone H11 unlabeled (Cat. No. MC-106), biotin-labeled (Cat. No. MC-126), or FITC-labeled (Cat. No. MC-136). [ Note: Activated T cells do not express high levels of surface Fas Ligand.] Western Blot: Excellent for this application. Use 2 µg/mL. Use unlabeled (Cat. No. MC-106) or biotin-labeled (Cat. No. MC-126). The optimal dilution for a specific application should be determined by the researcher. Figure 1. Immunocytochemistry: Detection of FasL in 293T cells transfected with a FasL expression vector. Method: Transfected cells were plated onto polylysine treated glass slides, fixed and permeabilized in methanol at -20 °C for 5 min, then in acetone at -20 °C for 30 sec. After 3 washes in PBS, 0.1% BSA, slides were incubated for 1 hr at RT with 20 µg/mL of biotinylated H11 antibody (MC-126) in PBS, 0.1% BSA. After rinsing in PBS, FITC-conjugated streptavidin was added for 30 min. Slides were washed again in PBS and visualized using a fluorescence microscope. Method: FasL transfected 293T cells (5x105) were incubated on ice for 30 min in 50 µL FACS buffer (PBS, 5% fetal calf serum, 0.02% sodium azide) containing 1 µg of FITC-labeled H11 antibody (MC-136). After washing in FACS buffer, cells were analyzed by flow cytometry. Do not exclude pre-apoptotic cells during data acquisition.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 µL of 1 mg/mL antibody in PBS with 0.02% sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Détails sur FASL

Antigène: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Autre désignation: CD178 / Fas Ligand

Sujet: Fas Ligand (FasL) is a type II membrane protein and a member of the tumor necrosis factor family. FasL is released by cleavage by metalloproteinases. When interacting with the Fas receptor, the trimeric FasL induces apoptosis. It is responsible for the activation-induced cell death of peripheral T cells and functions as an effector molecule for cytotoxic T cells. FasL is expressed in activated lymphocytes, Sertoli cells, and in certain tumor cells.

Pathways: Apoptose, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity