APAF1 anticorps

N° du produit ABIN187407

Cet anticorps anti-APAF1 est un anticorps Rat Monoclonal détectant APAF1 dans WB, IP, IF et IHC (fro). Adapté pour Humain.

Aperçu rapide pour APAF1 anticorps (ABIN187407)

Antigène: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Reactivité: Humain

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp APAF1 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 2E12

Détail du produit anti-APAF1 anticorps

Specificité: Recognizes the CARD domain of human Apaf-1. Species Reactivity: Human, others not tested. Does not cross-react with mouse or rat Apaf-1.

Immunogène: FLAG-tagged recombinant human Apaf-1 (aa 1-464) containing the N-terminal CARD and CED-4 homologous domains.

Isotype: IgG2a

Information d'application

Indications d'application: Immunocytochemistry Immunohistochemistry: Electron microscopy on frozen sections. Immunoprecipitation Western blot ELISA The optimal dilution for a specific application should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL.

Buffer: 100 µg of liquid Protein G purified monoclonal antibody in PBS containing 0.02% sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Détails sur APAF1

Antigène: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Autre désignation: APAF1

Sujet: Programmed cell death (apoptosis) requires the activation of caspases, which are in turn regulated by adaptor proteins such as Apaf-1 (activator of apoptosis protease-activating factor-1). The 136 kDa protein Apaf-1 is a mammalian homolog of C. elegans/CED-4, and is required for some pathways of cell death. Apaf-1 promotes the aggregation and self-processing of the initiator caspase, caspase-9 in the presence of cytochrome c and dATP. It consists of four function-determining regions: (1) an N-terminal caspase activation and recruitment domain (CARD), which is required for binding to pro-caspase-9, (2) a central CED-4 homologous region, (3) an Apaf-1 specific region, and (4) 12 identified WD40 repeats at the C-terminus of the protein.

Pathways: Signalisation p53, Apoptose, Caspase Cascade in Apoptosis, Tube Formation, Positive Regulation of Endopeptidase Activity