APAF1 anticorps

N° du produit ABIN187407

Cet anticorps anti-APAF1 Monoclonal Rat (Clone 2E12) (ABIN187407) détecte spécifiquement APAF1 dans WB, IF, IP et IHC (fro). L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour APAF1 anticorps (ABIN187407)

Antigène: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Reactivité: Humain

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp APAF1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 2E12

Détail du produit anti-APAF1 anticorps

Specificité: Recognizes the CARD domain of human Apaf-1. Species Reactivity: Human, others not tested. Does not cross-react with mouse or rat Apaf-1.

Immunogène: FLAG-tagged recombinant human Apaf-1 (aa 1-464) containing the N-terminal CARD and CED-4 homologous domains.

Isotype: IgG2a

Information d'application

Indications d'application: Immunocytochemistry Immunohistochemistry: Electron microscopy on frozen sections. Immunoprecipitation Western blot ELISA The optimal dilution for a specific application should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL.

Buffer: 100 µg of liquid Protein G purified monoclonal antibody in PBS containing 0.02% sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Détails sur APAF1

Antigène: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Autre désignation: APAF1

Sujet: Programmed cell death (apoptosis) requires the activation of caspases, which are in turn regulated by adaptor proteins such as Apaf-1 (activator of apoptosis protease-activating factor-1). The 136 kDa protein Apaf-1 is a mammalian homolog of C. elegans/CED-4, and is required for some pathways of cell death. Apaf-1 promotes the aggregation and self-processing of the initiator caspase, caspase-9 in the presence of cytochrome c and dATP. It consists of four function-determining regions: (1) an N-terminal caspase activation and recruitment domain (CARD), which is required for binding to pro-caspase-9, (2) a central CED-4 homologous region, (3) an Apaf-1 specific region, and (4) 12 identified WD40 repeats at the C-terminus of the protein.

Pathways: Signalisation p53, Apoptose, Caspase Cascade in Apoptosis, Tube Formation, Positive Regulation of Endopeptidase Activity