OGT anticorps (Internal Region)

N° du produit ABIN190863

Cet anticorps anti-OGT Polyclonal Chèvre (ABIN190863) détecte spécifiquement OGT dans WB, IHC, ELISA, IF et FACS. L’anticorps est réactif avec des échantillons de Humain et Rat.

Aperçu rapide pour OGT anticorps (Internal Region) (ABIN190863)

Antigène: OGT (O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT))

Reactivité: Humain, Rat

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp OGT est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Classe de qualité: Verified

Détail du produit anti-OGT anticorps

Épitope: Internal Region

Fonction: OGT

Séquence: YEHPKDLKLS DGR

Specificité: This antibody is expected to recognise both reported isoforms (NP_858058.1 and NP_858059.1

Réactivité croisée: Boeuf (Vache), Chien, Humain, Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-YEHPKDLKLSDGR, from the internal region of the protein sequence according to NP_858058.1, NP_858059.1.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: Paraffin embedded Human Brain (Cortex). Recommended concentration: 5 μg/mL.

Western Blot: Approx 110 kDa band observed in Rat Pancreas lysates calculated MW of 116 kDa according to NP_858058.2). An additional band of unknown identity was also consistently observed at 60 kDa. This band was successfully blocked by incubation with the

Peptide ELISA: antibody detection limit dilution 1:64000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the nucleus of HeLa, U2OS and Glioblastoma U251 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recomme

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur OGT

Antigène: OGT (O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT))

Autre désignation: OGT

Sujet: OGT, O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase), FLJ23071, HRNT1, MGC22921, O-GLCNAC , O-GlcNAc transferase p110 subunit O-linked GlcNAc transferase, uridinediphospho-N-acetylgl

ID gène: 8473, 108155, 26295

NCBI Accession: NP_858058, NP_858059

Pathways: Regulation of Carbohydrate Metabolic Process