H2AFX anticorps (pSer139)

N° du produit ABIN197033

Cet anticorps Lapin Polyclonal détecte spécifiquement H2AFX dans WB et IF. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour H2AFX anticorps (pSer139) (ABIN197033)

Antigène: H2AFX (H2A Histone Family, Member X (H2AFX))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp H2AFX est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Détail du produit anti-H2AFX anticorps

Épitope: pSer139

Specificité: This antibody detects endogenous levels of Histone H2A.X only when phosphorylated at Serine 139.

Purification: Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Immunogène: The antiserum was produced against synthesized phosphopeptide derived from human Histone H2A.X around the phosphorylation site of serine 139 (Q-A-SP-Q-E).

Information d'application

Indications d'application: Western Blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the antibody (in aliquots) at -20 °C.

Détails sur H2AFX

Antigène: H2AFX (H2A Histone Family, Member X (H2AFX))

Autre désignation: Histone H2A.x

Sujet: Histone H2A.X is a member of the histone H2A family, 1 of 5 families of histone proteins involved in nucleosomal organization of chromatin. Histone H2A.X is synthesised in G1 as well as S phase and is known to be important for recombination between immunoglobulin switch regions. A very early step in the response of mammalian cells to DNA double strand breaks is the phosphorylation of Histone H2A.X at serine 139 at the sites of DNA damage. There is a predicted acetylation at residue 1 and ubiquitination at reside 119. Phosphorylated Histone H2A.X promotes DNA repair and maintains genomic stability.Synonyms: H2AFX, H2AX, H2a/x

ID gène: 3014

NCBI Accession: NP_002096

UniProt: P16104

Pathways: Telomere Maintenance, Réparation de l'ADN, Positive Regulation of Response to DNA Damage Stimulus