H2AFX anticorps (Ser139)

N° du produit ABIN197524

Cet anticorps Lapin Polyclonal détecte spécifiquement H2AFX dans WB, IF et IHC (p). Il présente une réactivité envers Humain.

Aperçu rapide pour H2AFX anticorps (Ser139) (ABIN197524)

Antigène: H2AFX (H2A Histone Family, Member X (H2AFX))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp H2AFX est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-H2AFX anticorps

Épitope: Ser139

Specificité: This antibody detects endogenous levels of total Histone H2A.X protein.

Purification: Affinity Chromatography using epitope-specific immunogen

Immunogène: The antiserum was produced against synthesized non-phosphopeptide derived from human Histone H2A.X around the phosphorylation site of serine 139 (Q-A-SP-Q-E).

Information d'application

Indications d'application: Western Blot: 1/500-1/1000. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % Sodium Azide as preservative and 50 % Glycerol as stabilizer.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the antibody undiluted at -20 °C.

Détails sur H2AFX

Antigène: H2AFX (H2A Histone Family, Member X (H2AFX))

Autre désignation: Histone H2A.x

Sujet: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. H2AX is an early responder to DNA damage, and its change to what is known as gamma-H2AX is important for the coordination of signaling and repair activities.Synonyms: H2AFX, H2AX, H2a/x

ID gène: 3014

NCBI Accession: NP_002096

UniProt: P16104

Pathways: Telomere Maintenance, Réparation de l'ADN, Positive Regulation of Response to DNA Damage Stimulus