PKC delta anticorps (AA 643-647)

N° du produit ABIN197564

L’anticorps anti-PKC delta Polyclonal Lapin est utilisé pour la détection de PKC delta dans des échantillons de Humain, Souris et Rat. Il a été validé pour WB, IF et IHC (p).

Aperçu rapide pour PKC delta anticorps (AA 643-647) (ABIN197564)

Antigène: PKC delta (PKCd) (Protein Kinase C, delta (PKCd))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PKC delta est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-PKC delta anticorps

Épitope: AA 643-647

Specificité: This antibody detects endogenous levels of total PKC-delta protein.

Purification: Immunoaffinity Chromatography using epitope-specific immunogen.

Immunogène: Peptide sequence around amino acids 643~647 (R-L-Sp-Y-S) derived from Human PKC- delta

Information d'application

Indications d'application: Western blot: 1/500approx. 1/1000. Immunofluorescence: 1/100approx. 1/200. Immunohistochemistry on Paraffin-Embedded Sections: 1/50approx. 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the antibody (in aliquots) at -20 °C.

Détails sur PKC delta

Antigène: PKC delta (PKCd) (Protein Kinase C, delta (PKCd))

Autre désignation: PRKCD

Sujet: Protein Kinase C delta (PKC delta) is a 78 kDa member of the novel group (nPKCs: sensitive to diacylglycerol, phosphatidylserine, and phorbol esters) of the PKC family of serine/threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation. PKC delta is an ubiquitously expressed PKC isozyme that has been implicated in the regulation of multiple cellular processes including cell cycle progression and apoptosis. Autophosphorylation of serine 664 (serine 662 in mouse and rat) contributes to PKC delta activity. Serum dependent phosphorylation of serine 664 is mediated by mTOR pathway, is protected from dephosphorylation by phosphorylated threonine 505 in the activation loop, and is implicated in prolactin signaling.Synonyms: PKC delta, PKC-delta, PKCD, Protein kinase C delta type

ID gène: 5580

NCBI Accession: NP_006245

UniProt: Q05655

Pathways: Interferon-gamma Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Myometrial Relaxation and Contraction, M Phase, G-protein mediated Events, Dicarboxylic Acid Transport, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, Lipid Metabolism