CD247 anticorps (pTyr153)

N° du produit ABIN1981867

Cet anticorps Souris Monoclonal détecte spécifiquement CD247 dans WB et FACS. Il présente une réactivité envers Humain et Souris.

Aperçu rapide pour CD247 anticorps (pTyr153) (ABIN1981867)

Antigène: CD247 (CD247 Molecule (CD247))

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD247 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS)

Clone: EM-17

Détail du produit anti-CD247 anticorps

Épitope: pTyr153

Fonction: Anti-Hu CD3 zeta (pY153) Purified

Specificité: The mouse monoclonal antibody EM-17 recognizes phosphorylated intracellular tyrosine 153 of CD3 zeta chain (CD247), which is a component of TCR/CD3 complex expressed on T cells.

Réactivité croisée (Details): Human, Mouse

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: A phospho specific peptide corresponding to the amino acids surrounding tyrosine 153 of mouse CD3 zeta linked to KLH

Isotype: IgG1

Information d'application

Indications d'application: Western blotting: Recommended dilution: 2 - 5 μg/mL, positive control: Jurkat cells lysate treated with pervanadate, splenocyte lysate of Balb/c or F1 mouse treated with pervanadate, non-reducing conditions recommended.
Flow cytometry: Intracellular staining, recommended dilution: 1-4 μg/mL, positive control: Jurkat cells treated with pervanadate.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze. Do not use after expiration date stamped on vial label.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Détails sur CD247

Antigène: CD247 (CD247 Molecule (CD247))

Autre désignation: CD3 zeta

Sujet: CD247 Molecule,CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta (CD247). These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.,CD3 zeta chain, CD247, T3Z, TCRz, IMD25

ID gène: 919

UniProt: P20963

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway