Early Protein anticorps (Biotin)

N° du produit ABIN1993428

Détail du produit

Antigène: Early Protein

Reactivité: Human Papillomavirus 16 (HPV-16)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Biotin

Application: ELISA, Western Blotting (WB)

Purification: Caprylic Acid Ammonium Sulfate Precipitation

Immunogène: Recombinant human papillomavirus type 16 Protein E6 protein

Isotype: IgG

Information d'application

Indications d'application: Optimal working concentration should be determinde by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 0.03 % Proclin 300, 53 % Glycerol, 0.01 M PBS, pH 7.4

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin^TM: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze.

Stock: -20 °C/-80 °C

Stockage commentaire: Upon delivery aliquot and store at -20 °C or -80 °C.

Détail du antigène

Antigène: Early Protein

Autre désignation: Protein E6

Synonymes: anticorps early protein 6, anticorps E6

Classe de substances: Viral Protein

Sujet: Plays a major role in the induction and maintenance of cellular transformation. Acts mainly as an oncoprotein by stimulating the destruction of many host cell key regulatory proteins. E6 associates with host E6-AP ubiquitin-protein ligase, and inactivates tumor suppressors TP53 and TP73 by targeting them to the 26S proteasome for degradation. In turn, DNA damage and chromosomal instabilities increase and lead to cell proliferation and cancer development. The complex E6/E6P targets several other substrates to degradation via the proteasome including host NFX1-91, a repressor of human telomerase reverse transcriptase (hTERT). The resulting increased expression of hTERT prevents the shortening of telomere length leading to cell immortalization. Other cellular targets including Bak, Fas-associated death domain-containing protein (FADD) and procaspase 8, are degraded by E6/E6AP causing inhibition of apoptosis. E6 also inhibits immune response by interacting with host IRF3 and TYK2. These interactions prevent IRF3 transcriptional activities and inhibit TYK2-mediated JAK-STAT activation by interferon alpha resulting in inhibition of the interferon signaling pathway.
Synonyms: E6

UniProt: P03126