CTNNB1 anticorps (C-Term)
Aperçu rapide pour CTNNB1 anticorps (C-Term) (ABIN200360)
Antigène
Voir toutes CTNNB1 AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Épitope
- C-Term
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Specificité
- Recognizes chicken b-Catenin protein (~92kD). Does not cross-react with the related 83kD g-catenin (plakoglobin) protein. Also specifically detects an additional 120kD protein of unknown identity in extracts derived from A431 cells. This band is not detected in lysates derived from HeLa cells or human fibroblasts. Species cross-reactivity: Human & mouse. Reactivity with rat is likely but not confirmed.
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Purification
- Protein A purified
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Immunogène
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Fusion protein consisting of the maltose binding protein fused to a 100 amino acid segment of the C-terminus of chicken b-Catenin.
Type of Immunogen: Fusion protein -
Isotype
- IgG1 kappa
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Indications d'application
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Approved: IF (5 - 10 μg/mL), IHC, IHC-P, IP, WB (1 μg/mL)
Usage: Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry. Immunofluorescence: 5-10 μg/mL. Western Blot: 1 μg/mL. Immunoprecipitation: 2-5 μg. Immunohistochemistry (Formalin-fixed and paraffin-embedded tissue): 2-10 μg/mL. Requires heat induced epitope retrieval. Positive control: HeLa, A431 and WI-38 human cell lines. -
Commentaires
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Target Species of Antibody: Chicken
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- Lot specific
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Buffer
- PBS, pH 7.4, 0.1 % sodium azide.
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Stock
- 4 °C,-20 °C
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Stockage commentaire
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Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.
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- CTNNB1 (Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1))
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Autre désignation
- CTNNB1 / Beta Catenin
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Sujet
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Name/Gene ID: CTNNB1
Family: Catenin
Synonyms: CTNNB1, Beta-catenin, Catenin beta-1, CTNNB, Beta Catenin -
ID gène
- 1499
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UniProt
- P35222
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Pathways
- Signalisation WNT, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Tube Formation, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2
Antigène
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