CTNNB1 anticorps (C-Term)

N° du produit ABIN200360

L’anticorps anti-CTNNB1 Monoclonal Souris est utilisé pour la détection de CTNNB1 dans des échantillons de Humain, Souris et Poulet. Il a été validé pour WB, IHC, IF, IHC (p) et IP.

Aperçu rapide pour CTNNB1 anticorps (C-Term) (ABIN200360)

Antigène: CTNNB1 (Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1))

Reactivité: Humain, Souris, Poulet

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CTNNB1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Détail du produit anti-CTNNB1 anticorps

Épitope: C-Term

Specificité: Recognizes chicken b-Catenin protein (~92kD). Does not cross-react with the related 83kD g-catenin (plakoglobin) protein. Also specifically detects an additional 120kD protein of unknown identity in extracts derived from A431 cells. This band is not detected in lysates derived from HeLa cells or human fibroblasts. Species cross-reactivity: Human & mouse. Reactivity with rat is likely but not confirmed.

Purification: Protein A purified

Immunogène: Fusion protein consisting of the maltose binding protein fused to a 100 amino acid segment of the C-terminus of chicken b-Catenin.

Type of Immunogen: Fusion protein

Isotype: IgG1 kappa

Information d'application

Indications d'application: Approved: IF (5 - 10 μg/mL), IHC, IHC-P, IP, WB (1 μg/mL)

Usage: Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry. Immunofluorescence: 5-10 μg/mL. Western Blot: 1 μg/mL. Immunoprecipitation: 2-5 μg. Immunohistochemistry (Formalin-fixed and paraffin-embedded tissue): 2-10 μg/mL. Requires heat induced epitope retrieval. Positive control: HeLa, A431 and WI-38 human cell lines.

Commentaires:

Target Species of Antibody: Chicken

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Détails sur CTNNB1

Antigène: CTNNB1 (Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1))

Autre désignation: CTNNB1 / Beta Catenin

Sujet: Name/Gene ID: CTNNB1
Family: Catenin

Synonyms: CTNNB1, Beta-catenin, Catenin beta-1, CTNNB, Beta Catenin

ID gène: 1499

UniProt: P35222

Pathways: Signalisation WNT, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Tube Formation, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2