Nestin anticorps

N° du produit ABIN201334

Cet anticorps Souris Monoclonal détecte spécifiquement Nestin dans WB, IHC, ICC et IHC (fro). Il présente une réactivité envers Souris et Rat.

Aperçu rapide pour Nestin anticorps (ABIN201334)

Antigène: Nestin (NES)

Reactivité: Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Nestin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-Nestin anticorps

Specificité: Recognizes rodent Nestin at 200-220kD. Specificity confirmed with mouse and rat. Does not cross-react with human.

Purification: Purified

Immunogène: Nestin purified from embryonic rat spinal cord.

Type of Immunogen: Purified protein

Isotype: IgG1

Information d'application

Indications d'application: Approved: ICC (1:20 - 1:200), IHC, IHC-Fr (1:10 - 1:100), WB

Usage: Suitable for use in Immunohistochemistry, Western Blot and Immunocytochemistry. Immunohistochemistry (frozen or paraffin): 1:20-1:200. Perfuse tissue with 4 % paraformaldehyde,  pH 7.4 for light microscopy or with either 4 % paraformaldehyde,  pH 10 or 4 % paraformaldehyde with 0.1 % glutaraldehyde,  pH 7.4 for EM. Immunocytochemistry: 1:20-1:200. Fix cells in 4 % paraformaldehyde buffered with 50 mM sodium borate,  pH 9.5. Western Blot: 200-220kD in reducing gels of newborn rat or mouse cell extracts. Prepare samples by boiling in 4 volumes of 125 mM Tris,  pH 6.8, 10 % 2-mercaptoethanol, 10 % glycerol, 4.6 % SDS. 5 % PAGE gels are suggested. Block membranes with milk or BSA. Do not dilute primary antibody in buffer containing milk. Freezing and thawing of samples or samples not stored at -70?C will show breakdown of nestin sometimes into multiple fragments. Positive control: Mouse embryonic brain tissue.

Not recommended for: IHC-P

Commentaires:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Tris-glycine, 0.15 M sodium chloride,  pH 7.4, 0.05 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur Nestin

Antigène: Nestin (NES)

Autre désignation: Nestin

Sujet: Name/Gene ID: NES

Synonyms: NES, Nestin, Nbla00170

ID gène: 10763

UniProt: P48681