EPH Receptor A2 anticorps (Extracellular Domain)

N° du produit ABIN204192

Cet anticorps anti-EPH Receptor A2 est un anticorps Souris Monoclonal détectant EPH Receptor A2 dans WB, FACS et ICC. Adapté pour Humain, Souris, Chien et Boeuf (Vache).

Aperçu rapide pour EPH Receptor A2 anticorps (Extracellular Domain) (ABIN204192)

Antigène: EPH Receptor A2 (EPHA2)

Reactivité: Humain, Souris, Chien, Boeuf (Vache)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp EPH Receptor A2 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Détail du produit anti-EPH Receptor A2 anticorps

Épitope: Extracellular Domain

Specificité: Recognizes human EphA2.

Purification: Protein G purified

Immunogène: NS0-derived rhEphA2 extracellular domain.

Isotype: IgG2a

Information d'application

Indications d'application: Approved: Flo (25 μg/mL), ICC (10 μg/mL), WB (1 - 2 μg/mL)

Usage: Suitable for use in Flow Cytometry, Western Blot and Immunocytochemistry. Flow Cytometry: 25 μg/mL. Add 10 μL to label 1-2.5x10^5 cells in a total reaction volume not exceeding 200 μL. Western Blot: 1-2 μg/mL. Using a colorimetric detection system, the detection limit for rhEphA2 is approximately 1 ng/lane under non-reducing conditions. Immunocytochemistry: 10 μg/mL to detect EphA2 on A431 cells. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by Rhodamine RedTM-coupled anti-mouse IgG at RT in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute with 200 μL sterile PBS.

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.4, 5 % trehalose

Conseil sur la manipulation: Avoid frequent freeze thaws. Store undiluted.

Stock: -20 °C,-80 °C

Stockage commentaire: Store at -20°C to -70°C. Stable for 1 year from date of receipt.

Détails sur EPH Receptor A2

Antigène: EPH Receptor A2 (EPHA2)

Autre désignation: EPHA2 / EPH Receptor A2

Sujet: Name/Gene ID: EPHA2
Subfamily: Ephrin Receptor
Family: Protein Kinase

Synonyms: EPHA2, CTPA, EPH receptor A2, Ephrin type-A receptor 2, Epithelial cell kinase, ECK, Sek-2, ARCC2, Soluble EPHA2 variant 1, CTPP1

ID gène: 1969

UniProt: P29317

Pathways: Signalisation RTK