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ITGB1 anticorps

Cet anticorps anti-ITGB1 est un anticorps Souris Monoclonal détectant ITGB1 dans FACS, ELISA, IHC, IP, IHC (p), ICC et IHC (fro). Adapté pour Humain.
N° du produit ABIN204312

Aperçu rapide pour ITGB1 anticorps (ABIN204312)

Antigène

Voir toutes ITGB1 Anticorps
ITGB1 (Integrin beta 1 (ITGB1))

Reactivité

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Humain

Hôte

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Souris

Clonalité

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Monoclonal

Conjugué

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Cet anticorp ITGB1 est non-conjugé

Application

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Flow Cytometry (FACS), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))
  • Specificité

    Recognizes human Integrin beta 1. Reacts with calcium-dependent epitopes on the integrin b1 subunit. When bound to b1, activates b1 dependent binding to extra cellular matrix substrates. Species cross-reactivity: Rabbit and monkey.

    Purification

    Protein A purified

    Immunogène

    Human IL-1b activated endothelial cells.

    Type of Immunogen: Cells

    Isotype

    IgG1
  • Indications d'application

    Approved: ELISA, Flo, ICC (2 μg/mL), IHC, IHC-Fr, IHC-P, IP

    Usage: Suitable for use in Immunohistochemistry, Immunocytochemistry, Immunoprecipitation, FACS and ELISA. Immunocytochemistry: 2 μg/mL (use on numerous cell lines as long as Ca2+ is present),. Immunoprecipitation: 5 μg/400 μL lysate (0.5 % triton PBS plus Ca++). FACS: 2-5 μg/1 x106, in the presence of Ca++. Stimulation of cells: 2-10 μg/mL per 1 x106. Binding buffer must contain 1 mM Ca++. Immunohistochemistry: For use on acetone or mild paraformaldehyde fixed tissue (2-2.5 % PFA, short time) frozen and paraffin embedded (1:500, citrate HEIR treated, In et al 2003). Ca++ required for binding. Ca++ must be used in blocking, washing, and antibody incubation buffers.

    Commentaires

    Target Species of Antibody: Human

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    PBS,  pH 7.1. No preservatives.

    Agent conservateur

    Without preservative

    Stock

    4 °C,-20 °C

    Stockage commentaire

    Short-term: 4°C, Long-term: Add sterile 40-50% glycerol, aliquot, and store at -20°C.
  • Antigène

    ITGB1 (Integrin beta 1 (ITGB1))

    Autre désignation

    ITGB1 / Integrin Beta 1 / CD29

    Sujet

    Name/Gene ID: ITGB1
    Family: Integrin

    Synonyms: ITGB1, CD29, CD29 antigen, Integrin beta-1, MDF2, MSK12, Integrin VLA-4 beta subunit, VLA-BETA, VLAB, Beta 1 integrin, FNRB, GPIIA, Integrin Beta 1, VLA-4 subunit beta

    ID gène

    3688

    UniProt

    P05556

    Pathways

    Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor, Integrin Complex, SARS-CoV-2 Protein Interactome
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