Nestin anticorps

N° du produit ABIN205661

L’anticorps Souris Monoclonal anti-Nestin a été validé pour WB, IHC et IHC (fro). Il convient pour détecter Nestin dans des échantillons de Souris et Rat.

Aperçu rapide pour Nestin anticorps (ABIN205661)

Antigène: Nestin (NES)

Reactivité: Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Nestin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: Rat-401-4D4

Détail du produit anti-Nestin anticorps

Specificité: Recognizes rat nestin, a large intermediate filament protein transiently expressed in embryonic glial cells. It is predominately expressed in stem cells of the developing nervous system. Terminal differentiation is associated with a loss of nestin expression. Nestin expression has also been noted in other embryonic tissues, also in most Glioblastoma multiformes and many melanomas.

Aucune reactivité croisée: Humain

Purification: Purified

Immunogène: Nestin purified from embryonic rat spinal cord.

Type of Immunogen: Purified protein

Isotype: IgG1

Information d'application

Indications d'application: Approved: IHC, IHC-Fr (1:20 - 1:200), WB

Usage: Immunohistochemistry: We recommend perfusing tissues with 4 % paraformaldehyde at  pH 7.4 for light microscopy or with either 4 % paraformaldehyde at  pH 10.0 or 4 % paraformaldehyde with 0.1 % glutaraldehyde at  pH 7.4 for EM. For Immunocytochemistry we recommend using cells fixed in 4 % paraformaldehyde buffered with 50 mM sodium borate at  pH 9.5. Western Blot: LS-C42054 reacts with a band at 200-220 kD in reducing gels of newborn rat or mouse cell extracts. For the purpose of western blotting it is recommended that samples should be boiled in 4 volumes of 125 mM Tris,  pH 6.8, 10 % 2-mercaptoethanol, 10 % glycerol and 4.6 % SDS. Membranes should be blocked with milk or BSA. 5 % PAGE gels are suggested.

Not recommended for: IHC-P

Commentaires:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: avoid freeze thaw cycles. Store undiluted.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.

Détails sur Nestin

Antigène: Nestin (NES)

Autre désignation: Nestin

Sujet: Name/Gene ID: NES

Synonyms: NES, Nestin, Nbla00170

ID gène: 10763