H2AFX anticorps (pSer139)

N° du produit ABIN208587

Cet anticorps anti-H2AFX est un anticorps Souris Monoclonal détectant H2AFX dans WB, IF, ICC et IP. Adapté pour Humain, Boeuf (Vache), Chien, Lapin et Singe.

Aperçu rapide pour H2AFX anticorps (pSer139) (ABIN208587)

Antigène: H2AFX (H2A Histone Family, Member X (H2AFX))

Reactivité: Humain, Boeuf (Vache), Chien, Lapin, Singe

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp H2AFX est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Détail du produit anti-H2AFX anticorps

Épitope: AA 134-142, pSer139

Specificité: Recognizes human Histone H2A. X phosphorylated at Ser139, Mr 15kD. A broad species cross-reactivity is expected based on conservation of sequence homology.

Purification: Protein G purified

Immunogène: Synthetic peptide (C-KATQA[pS]QEY) corresponding to aa134-142 of human histone H2A. X. (KLH). The immunizing sequence has 8 identical amino acids in yeast and mouse.

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotype: IgG1

Information d'application

Indications d'application: Approved: ICC (2 μg/mL), IF, IP, WB

Usage: Suitable for use in Western Blot, Immunofluorescence, Chromatin Immunoprecipitation and Immunocytochemistry. Proteins were visualized using a IgG (HRP) GtxMo and a chemiluminescence detection system. Immunocytochemistry: 2 μg/mL detects phosphorylated histone H2A. X (Ser139) in HeLa cells treated with 0.5 uM staurosporine for 4-6 hours. Positive control: UV-treated 293 cell extracts, UV-treated HeLa cell extracts, breast cancer tissue.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.6, 0.07 % sodium azide, 30 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur H2AFX

Antigène: H2AFX (H2A Histone Family, Member X (H2AFX))

Autre désignation: H2AX

Sujet: Name/Gene ID: H2AFX

Synonyms: H2AFX, H2AX, Histone H2A.x, H2A.X, H2A histone family, member X, H2AX histone

ID gène: 3014

UniProt: P16104

Pathways: Telomere Maintenance, Réparation de l'ADN, Positive Regulation of Response to DNA Damage Stimulus