IRS1 anticorps (AA 1222-1235)

N° du produit ABIN208882

Cet anticorps Lapin Polyclonal détecte spécifiquement IRS1 dans WB et IP. Il présente une réactivité avec des échantillons de Rat.

Aperçu rapide pour IRS1 anticorps (AA 1222-1235) (ABIN208882)

Antigène: IRS1 (Insulin Receptor Substrate 1 (IRS1))

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp IRS1 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP)

Détail du produit anti-IRS1 anticorps

Épitope: AA 1222-1235

Specificité: Recognizes IRS-1 at 160-185kD. Species cross-reactivity: Human, mouse and rat.

Homologie: Percent identity by BLAST analysis: Rat (100%) Human, Gorilla, Mouse, Hamster, Rabbit (93%) Marmoset, Chicken (86%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide corresponding to 14 amino acid ([C]YASINFQKQPEDRQ) with in the carboxy terminal of rat liver IRS-1. Percent identity by BLAST analysis: Rat (100%), Human, Gorilla, Mouse, Hamster, Rabbit (93%), Marmoset, Chicken (86%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IP, WB (0.5 - 2 μg/mL)

Usage: Suitable for use in Western Blot and Immunoprecipitation. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Previous lots detected IRS-1 in RIPA lysates of rat L6 myoblasts. In cells that contain high levels of IRS-1, this lot may work optimally at 0.1-0.5 μg/mL. Immunoprecipitation: 4 μg immunoprecipitates IRS-1 from 500 μg of 3T3/A31 RIPA lysate. Positive control: Fibroblast Cell, 3T3/A31, L6 myoblasts.

Commentaires:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: 0.2 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.1 mM EDTA, 5 mg/mL BSA, 0.05 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Détails sur IRS1

Antigène: IRS1 (Insulin Receptor Substrate 1 (IRS1))

Autre désignation: IRS1 / IRS-1

Sujet: Name/Gene ID: IRS1

Synonyms: IRS1, IRS-1, HIRS-1, Insulin receptor substrate 1

ID gène: 3667

UniProt: P35568

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Negative Regulation of Hormone Secretion, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process