MMP14 anticorps (AA 319-333)

N° du produit ABIN209030

L’anticorps Souris Monoclonal anti-MMP14 a été validé pour WB, ELISA, IHC, IP, IHC (p) et IHC (fro). Il convient pour détecter MMP14 dans des échantillons de Humain, Souris, Rat, Boeuf (Vache), Singe, Chévre, Hamster et Mouton.

Aperçu rapide pour MMP14 anticorps (AA 319-333) (ABIN209030)

Antigène: MMP14 (Matrix Metallopeptidase 14 (Membrane-inserted) (MMP14))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Singe, Chévre, Hamster, Mouton

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MMP14 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-MMP14 anticorps

Épitope: AA 319-333

Specificité: Recognizes human MT1-MMP. Very slight cross-reactivity to hMMP-3. Species cross-reactivity: Mouse, rat, rabbit porcine and equine. For unknown reasons this antibody does not react on westerns to MMP-14 [MT1-MMP], Human, Prodomain, Catalytic Domain.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Sheep, Goat, Hamster, Porcine, Bovine, Opossum, Platypus (100%) Elephant, Panda, Stickleback, Turkey, Bat, Dog, Horse, Rabbit, Chicken, Pufferfish (93%) Zebrafish, Xenopus, Medaka (87%) Lizard (80%).

Purification: Immunoaffinity purified

Immunogène: Peptide (aa 319-333, CDGNFDTVAMLRGEM) in the hemopexin-like domain of human membrane Type1 matrix metalloproteinase human MT1-MMP also referred to as MMP-14. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Sheep, Goat, Hamster, Porcine, Bovine, Opossum, Platypus (100%), Elephant, Panda, Stickleback, Turkey, Bat, Dog, Horse, Rabbit, Chicken, Pufferfish (93%), Zebrafish, Xenopus, Medaka (87%), Lizard (80%).

Isotype: IgG3 kappa

Information d'application

Indications d'application: Approved: ELISA, IHC, IHC-Fr, IHC-P, IP, WB (10 μg/mL)

Usage: Suitable for use in ELISA, Western Blot and Immunohistochemistry, and Immunoprecipitation. Western Blot: 10 μg/mL, membrane preparations, MD-MB-231 cells pretreated with 1-5 μg/mL ConA for 24-48 hours for positive control. 60-65kD proteins are identified. When tested against recombinant MT1-MMP (lacking the TM domain) expressed in CHO cells the antibody reacts with a band of ~59kD. Immunohistochemistry: Frozen sections, traditional formalin fixation problematic. Paraffin-embedded tissue sections with PLP fixation required.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.0, 2 % BSA (protease free).

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur MMP14

Antigène: MMP14 (Matrix Metallopeptidase 14 (Membrane-inserted) (MMP14))

Autre désignation: MMP14 / MMP-14

Sujet: Name/Gene ID: MMP14
Subfamily: Metallopeptidase M10A
Family: Protease

Synonyms: MMP14, Membrane type-1 MMP, MT1-MMP, MT1MMP, MT-MMP, MT-MMP 1, Matrix metalloproteinase-14, MMP-14, MMP-X1, MTMMP1

ID gène: 4323

UniProt: P50281

Pathways: Autophagy