STAT5B anticorps (AA 774-787)

N° du produit ABIN210645

Détail du produit anti-STAT5B anticorps

Antigène: STAT5B (Signal Transducer and Activator of Transcription 5B (STAT5B))

Épitope: AA 774-787

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Chien, Cheval, Porc, Hamster

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp STAT5B est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP)

Specificité: Recognizes the 90-92kD forms of STAT5B protein. Species cross-reactivity: Human and mouse.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Horse, Pig (100%) Panda, Bat, Chicken, Xenopus (93%) Opossum (86%).

Purification: Protein A purified

Immunogène: Amino acids 774-787 of human STAT5B (GRPMDSQWIPHAQS). The peptide was coupled to KLH prior to immunization. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Horse, Pig (100%), Panda, Bat, Chicken, Xenopus (93%), Opossum (86%).

Isotype: IgG

Information d'application

Indications d'application: Approved: IP, WB

Usage: Western Blot: 0.5-2 mg/mL detects STAT5B (doublet of 90kD and 92kD) in. human A431 and mouse 3T3 fibroblasts using enhanced chemiluminescence with a five minute exposure. Immunoprecipitation: 4 μg immunoprecipitates STAT5B from A431 carcinoma cells and a. previous lot immunoprecipitated STAT5B from human Raji cells. A RIPA lysate of human A431 cells (500 μg protein) was incubated with 4 μg of anti-STAT 5B Immune complexes were precipitated with protein A agarose and analyzed by immunoblotting with anti- STAT 5B. Bands were visualized using a goat anti-rabbit IgG secondary antibody conjugated to HRP in conjunction with enhanced chemiluminescence detection. Positive Antigen Control: mouse 3T3 cell lysate. Add 2.5 μL of 2-mercaptoethanol. per 100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as a liquid in 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Détails sur STAT5B

Antigène: STAT5B (Signal Transducer and Activator of Transcription 5B (STAT5B))

Autre désignation: STAT5B / STAT (STAT5B Produits)

Synonymes: anticorps STAT5, anticorps stat5, anticorps STAT5A, anticorps STAT5B, anticorps signal transducer and activator of transcription 5B, anticorps signal transducer and activator of transcription 5B S homeolog, anticorps Signal transducer and activator of transcription 5B, anticorps STAT5B, anticorps Stat5b, anticorps stat5b.S, anticorps LOC490969, anticorps sta5b, anticorps LOC100566752, anticorps LOC100052954

Sujet: Name/Gene ID: STAT5B

Synonyms: STAT5B, Transcription factor STAT5B, STAT5

ID gène: 6777

UniProt: P51692

Pathways: Signalistation JAK/STAT, Signalisation RTK, Response to Growth Hormone Stimulus, C21-Steroid Hormone Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events, Activated T Cell Proliferation