CHEK2 anticorps

N° du produit ABIN210859

Cet anticorps Souris Monoclonal détecte spécifiquement CHEK2 dans WB, IP et ICC. Il présente une réactivité envers Humain, Souris et Rat.

Aperçu rapide pour CHEK2 anticorps (ABIN210859)

Antigène: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CHEK2 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Détail du produit anti-CHEK2 anticorps

Specificité: Recognizes human CHK2, Mr 67kD. Species cross-reactivity: mouse. Species sequence homology: rat.

Purification: Protein G purified

Immunogène: Mixture of two GST fusion proteins corresponding to amino acids NT, 1-223 and CT 217-543 of human CHK2.

Type of Immunogen: Fusion protein

Isotype: IgG1

Information d'application

Indications d'application: Approved: ICC, IP, WB (0.1 - 1 μg/mL)

Usage: Suitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry. Immunoprecipitation: Detects HK2 from HeLa cells. Reported by an independent lab. Immunocytochemistry: Detects CHK2 in HeLa cells. Reported by an independent lab. Western Blot: 0.1-1.0 μg/mL detects CHK2 in lysates from Jurkat, HeLa, 3T3/A31, and A431 cells. Jurkat cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-CHK2 (0.5 μg/mL). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Positive control: J9000: Jurkat cell lysate. Add 2.5 μL of 2-mercaptoethanol/ 100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Détails sur CHEK2

Antigène: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Autre désignation: CHEK2 / CHK2

Sujet: Name/Gene ID: CHEK2
Subfamily: CDS1
Family: Protein Kinase

Synonyms: CHEK2, BA444G7, Cds1 homolog, Checkpoint kinase 2, Checkpoint-like protein CHK2, CHK2 checkpoint homolog, CHK2, HCds1, LFS2, RAD53, Rad53 homolog, HCHK2, HuCds1, PP1425

ID gène: 11200

UniProt: O96017

Pathways: Signalisation p53, Apoptose, Cycle Cellulaire