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PKC gamma anticorps (AA 351-614)

L’anticorps Souris Monoclonal anti-PKC gamma a été validé pour WB, IHC, IP et ICC. Il convient pour détecter PKC gamma dans des échantillons de Rat.
N° du produit ABIN2118965

Aperçu rapide pour PKC gamma anticorps (AA 351-614) (ABIN2118965)

Antigène

Voir toutes PKC gamma (PRKCG) Anticorps
PKC gamma (PRKCG) (Protein Kinase C, gamma (PRKCG))

Reactivité

  • 91
  • 78
  • 54
  • 4
  • 4
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
Rat

Hôte

  • 120
  • 5
  • 1
Souris

Clonalité

  • 111
  • 15
Monoclonal

Conjugué

  • 44
  • 10
  • 9
  • 6
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
Cet anticorp PKC gamma est non-conjugé

Application

  • 99
  • 41
  • 39
  • 39
  • 20
  • 14
  • 10
  • 9
  • 7
  • 5
  • 2
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
  • Épitope

    • 16
    • 15
    • 15
    • 8
    • 8
    • 6
    • 5
    • 5
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 351-614

    Fonction

    Monoclonal Antibody to Protein Kinase C Gamma (PKCg)

    Séquence

    MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- FSFLMVLGKG SFGKVMLAER RGSDELYAIK ILKKDVIVQD DDVDCTLVEK RVLALGGRGP GGRPHFLTQL HSTFQTPDRL YFVMEYVTGG DLMYHIQQLG KFKEPHAAFY AAEIAIGLFF LHNQGIIYRD LKLDNVMLDA EGHIKITDFG MCKENVFPGS TTRTFCGTPD YIAPEIIAYQ PYGKSVDWWS FGVLLYEMLA GQPPFDGEDE EELFQAIMEQ TVTYPKSLSR EAVAICKGFL TKHPGKRLGS GPDGEPTIRA HGFF

    Specificité

    The antibody is a mouse monoclonal antibody raised against PKCg. It has been selected for its ability to recognize PKCg in immunohistochemical staining and western blotting.

     Réactivité croisée

    Humain, Souris

    Purification

    Antigen-specific affinity chromatography followed by Protein A affinity chromatography

    Immunogène

    PKCg (AA 351-614)

    Isotype

    IgG
  • Indications d'application

    Western blotting: 0.2-2 μg/mL,1:500-5000 Immunohistochemistry: 5-20 μg/mL,1:50-200 Immunocytochemistry: 5-20 μg/mL,1:50-200 Optimal working dilutions must be determined by end user.

    Commentaires

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

    Conseil sur la manipulation

    Avoid repeated freeze/thaw cycles

    Stock

    4 °C,-20 °C

    Stockage commentaire

    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

    Date de péremption

    12 months
  • Antigène

    PKC gamma (PRKCG) (Protein Kinase C, gamma (PRKCG))

    Autre désignation

    PKCg

    Sujet

    PKC-G, PRKCG, PKCC, SCA14, PKC-Gamma

    Pathways

    Signalisation WNT, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Myometrial Relaxation and Contraction, G-protein mediated Events, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGF Signaling
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