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EPH Receptor A2 anticorps

Cet anticorps Souris Monoclonal détecte spécifiquement EPH Receptor A2 dans WB, IP, ICC et Func. Il présente une réactivité envers Humain, Souris, Rat, Chien et Boeuf (Vache).
N° du produit ABIN211915

Aperçu rapide pour EPH Receptor A2 anticorps (ABIN211915)

Antigène

Voir toutes EPH Receptor A2 (EPHA2) Anticorps
EPH Receptor A2 (EPHA2)

Reactivité

  • 113
  • 71
  • 46
  • 14
  • 5
  • 5
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Humain, Souris, Rat, Chien, Boeuf (Vache)

Hôte

  • 117
  • 19
  • 4
  • 2
Souris

Clonalité

  • 113
  • 28
  • 1
Monoclonal

Conjugué

  • 77
  • 9
  • 8
  • 7
  • 7
  • 6
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp EPH Receptor A2 est non-conjugé

Application

  • 120
  • 73
  • 28
  • 27
  • 17
  • 14
  • 12
  • 12
  • 7
  • 7
  • 6
  • 4
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Functional Studies (Func)
  • Specificité

    Specific for Epithelial Cell Kinase (Eck) at 140kD. Species cross-reactivity: Human, mouse, rat, bovine and canine.

    Purification

    Protein G purified

    Immunogène

    Native protein isolated by purification of phosphotyrosine-containing proteins.

    Isotype

    IgG1
  • Indications d'application

    Approved: Func, ICC, IP, WB

    Usage: Suitable for use in Western Blot, Protein Kinase Assay, Immunoprecipitation and Immunocytochemistry. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Protein Kinase Assay: Reported to have been used in an IP autophosphorylation assay, using a Mn-PIPES reaction buffer. Immunoprecipitation: Reported to immunoprecipitate Eck from 500 μg of a human breast epithelial cell line which had been lysed in TBS containing 1 % Triton X-100. Use 1-4 μg per reaction. Immunocytochemistry: Reported to immunostain Eck in human, mouse and rat epithelial cells fixed with 3.7 % formaldehyde solution and permeabilized with 0.5 % Triton X-100 in TBS.

    Commentaires

    Target Species of Antibody: Human

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    0.2 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Conseil sur la manipulation

    Avoid repeat freeze-thaw cycles.

    Stock

    4 °C,-20 °C

    Stockage commentaire

    Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
  • Antigène

    EPH Receptor A2 (EPHA2)

    Autre désignation

    EPHA2 / EPH Receptor A2

    Sujet

    Name/Gene ID: EPHA2
    Subfamily: Ephrin Receptor
    Family: Protein Kinase

    Synonyms: EPHA2, CTPA, EPH receptor A2, Ephrin type-A receptor 2, Epithelial cell kinase, ECK, Sek-2, ARCC2, Soluble EPHA2 variant 1, CTPP1

    ID gène

    1969

    UniProt

    P29317

    Pathways

    Signalisation RTK
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