Paxillin anticorps

N° du produit ABIN212824

Cet anticorps Souris Monoclonal détecte spécifiquement Paxillin dans WB, ELISA, IP et ICC. Il présente une réactivité envers Humain, Souris, Rat, Poulet et Boeuf (Vache).

Aperçu rapide pour Paxillin anticorps (ABIN212824)

Antigène: Paxillin (PXN)

Reactivité: Humain, Souris, Rat, Poulet, Boeuf (Vache)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Paxillin est non-conjugé

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC)

Détail du produit anti-Paxillin anticorps

Specificité: Recognizes human Paxillin at 68kD and a band around 50kD, which is most likely a degradation product. Species cross-reactivity: Human, mouse, rat, chicken and bovine. Other species not tested.

Purification: Protein G purified

Immunogène: GST fusion protein containing full-length human paxillin.

Type of Immunogen: Fusion protein

Isotype: IgG1

Information d'application

Indications d'application: Approved: ELISA, ICC (5 - 10 μg/mL), IP, WB (0.5 - 2 μg/mL)

Usage: Suitable for use in ELISA, Western Blot, Immunoprecipitation, and Immunocytochemistry. Western Blot: 0.5-2 μg/mL detects paxillin in RIPA lysates from 3T3 fibroblasts using 20 μg per lane for minigels. Immunoprecipitation: 4 μg per 500 μg of cell lysate. Immunocytochemistry:5-10 μg/mL (gave positive immunostaining for paxillin in REF (rat embryo fibroblast) 52 cells fixed with 3.7 % paraformaldehyde for 5 minutes).

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Détails sur Paxillin

Antigène: Paxillin (PXN)

Autre désignation: PXN / Paxillin

Sujet: Name/Gene ID: PXN

Synonyms: PXN, Paxillin

ID gène: 5829

UniProt: P49023

Pathways: Signalisation MAPK, EGFR Signaling Pathway, Response to Growth Hormone Stimulus, Cell-Cell Junction Organization, Maintenance of Protein Location, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling