CD48 anticorps (PE)
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- Antigène Voir toutes CD48 Anticorps
- CD48
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp CD48 est conjugé à/à la PE
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Application
- Flow Cytometry (FACS)
- Attributs du produit
- 4A156-4H9 reacts with blast-1, a 45 kDa GPI linked cell surface molecule. CD48 is expressed on peripheral blood lymphocytes, monocytes, or macrophages, but not on granulocytes and platelets nor on non-hematopoietic cells. CD48 binds to CD2 and plays a role as an accessory molecule in g/d T cell recognition and a/b T cell antigen recognition.* CD48 positive lymphocytes is decreased in patients with PNH (paroxysmal nocturnal haemoglobinuria)
- Clone
- 4A156-4H9
- Isotype
- IgG1
- Top Product
- Discover our top product CD48 Anticorps primaire
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- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Procédure de l'essai
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- Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
- Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
- Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
- Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
- [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
- [Cell fixation: 2 % formaldehyde solution]
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C
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- Antigène
- CD48
- Autre désignation
- CD48 (CD48 Produits)
- Synonymes
- anticorps AI449234, anticorps AW610730, anticorps BCM1, anticorps BLAST, anticorps BLAST-1, anticorps BLAST1, anticorps Bcm-1, anticorps MEM-102, anticorps SLAMF2, anticorps Sgp-60, anticorps hCD48, anticorps mCD48, anticorps CD48 antigen, anticorps Cd48 molecule, anticorps CD48 molecule, anticorps Cd48, anticorps CD48
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