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GPR84 anticorps (AA 21-120)

Name: GPR84 anticorps (AA 21-120)
SKU: ABIN2173922
Price: 414.62 EUR
Availability: InStock

GPR84 Reactivité: Humain, Souris, Lapin WB, ELISA, FACS, IHC (p), ICC, IF (cc), IF (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated

Mouse heart lysates probed with GPCR EX33 Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C. (GPR84 anticorps (AA 21-120))

Mouse bone and brain lysates probed with GPCR EX33 Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C. (GPR84 anticorps (AA 21-120))

Paraformaldehyde-fixed, paraffin embedded human glioma tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with GPCR EX33 Polyclonal Antibody, Unconjugated at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining (GPR84 anticorps (AA 21-120))

IHC (p)

Paraformaldehyde-fixed, paraffin embedded human colon carcinoma, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with GPCR EX33 Polyclonal Antibody, Unconjugated at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining. (GPR84 anticorps (AA 21-120))

IHC (p)

Mouse thymus lysates probed with GPCR EX33 Polyclonal Antibody, Unconjugated (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange). (GPR84 anticorps (AA 21-120))

FACS

5 images

N° du produit ABIN2173922

Fiche de données

Antigène Voir toutes GPR84 Anticorps

GPR84 (G Protein-Coupled Receptor 84 (GPR84))
Épitope
16

7

5

5

5

5

3

2

2

2

1

1

1

1

1

1

1

1

1
AA 21-120
Reactivité
44

25

8

4

4

2

1

1

1

1
Humain, Souris, Lapin
Hôte
58

3
Lapin
Anticorps secondaires appropriés
Clonalité
59

2
Polyclonal
Conjugué
20

6

4

4

4

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1
Cet anticorp GPR84 est non-conjugé

Application

Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Réactivité croisée

Humain, Souris, Lapin

Homologie

Cow,Pig

Purification

Purified by Protein A.

Immunogène

KLH conjugated synthetic peptide derived from human GPCR EX33 protein

Isotype

IgG
anti-G Protein-Coupled Receptor 84 (GPR84) (Cytoplasmic Domain) antibody
GPR84 Reactivité: Humain ELISA, IHC, IHC (p) Hôte: Lapin Polyclonal unconjugated

anti-G Protein-Coupled Receptor 84 (GPR84) (AA 208-316) antibody
GPR84 Reactivité: Humain WB, ELISA Hôte: Souris Monoclonal 5C3 unconjugated

anti-G Protein-Coupled Receptor 84 (GPR84) (2nd Extracellular Loop), (AA 166-180) antibody
GPR84 Reactivité: Humain, Souris, Rat WB, FACS, IHC, IF Hôte: Lapin Polyclonal unconjugated

anti-G Protein-Coupled Receptor 84 (GPR84) (Extracellular Domain) antibody
GPR84 Reactivité: Humain, Cheval, Singe IHC, IHC (p) Hôte: Lapin Polyclonal unconjugated

anti-G Protein-Coupled Receptor 84 (GPR84) (Transmembrane Domain) antibody
GPR84 Reactivité: Humain IHC, IHC (p) Hôte: Lapin Polyclonal unconjugated

anti-G Protein-Coupled Receptor 84 (GPR84) (N-Term) antibody
GPR84 Reactivité: Humain, Souris WB, ELISA, IHC Hôte: Lapin Polyclonal unconjugated

anti-G Protein-Coupled Receptor 84 (GPR84) antibody
GPR84 Reactivité: Souris, Rat WB Hôte: Lapin Polyclonal unconjugated

anti-G Protein-Coupled Receptor 84 (GPR84) (AA 202-320) antibody
GPR84 Reactivité: Humain ELISA, IF Hôte: Lapin Polyclonal unconjugated

anti-G Protein-Coupled Receptor 84 (GPR84) (AA 1-100) antibody
GPR84 Reactivité: Souris WB, ELISA, FACS Hôte: Lapin Polyclonal unconjugated

anti-G Protein-Coupled Receptor 84 (GPR84) (AA 265-293) antibody
GPR84 Reactivité: Humain WB Hôte: Lapin Polyclonal RB36053 unconjugated

Indications d'application

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Agent conservateur

ProClin

Précaution d'utilisation

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock

4 °C,-20 °C

Stockage commentaire

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption

12 months
Antigène

GPR84 (G Protein-Coupled Receptor 84 (GPR84))

Autre désignation

GPCR EX33 (GPR84 Produits)

Synonymes

anticorps EX33, anticorps GPCR4, anticorps GPR84, anticorps sb:eu355, anticorps zgc:171802, anticorps G protein-coupled receptor 84, anticorps G protein-coupled receptor 84 S homeolog, anticorps GPR84, anticorps Gpr84, anticorps gpr84, anticorps gpr84.S

Sujet

Synonyms: EX33, GPCR4, G-protein coupled receptor 84, Inflammation-related G-protein coupled receptor EX33, GPR84
Background: Receptor for medium-chain free fatty acid (FFA) with carbon chain lengths of C9 to C14. Capric acid (C10:0), undecanoic acid (C11:0) and lauric acid (C12:0) are the most potent agonists. Not activated by short-chain and long-chain saturated and unsaturated FFAs. Activation by medium-chain free fatty acid is coupled to a pertussis toxin sensitive G(i/o) protein pathway. May have important roles in processes from fatty acid metabolism to regulation of the immune system.

ID gène

53831

UniProt

Q9NQS5