CD36 anticorps

N° du produit ABIN2191823

Détail du produit anti-CD36 anticorps

Antigène: CD36

Reactivité: Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD36 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Functional Studies (Func)

niveau d'endotoxine: Low endotoxin level

Clone: CRF D-2712

Information d'application

Indications d'application: For flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:25. For functional studies dilutions have to be made according to the amounts of CD36 binding to be blocked.

Restrictions: For Research Use only

Stockage

Buffer: 2 mL(> 100 μg/mL) culture medium with low endotoxin level.

Stock: 4 °C

Stockage commentaire: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Date de péremption: 12 months

Références pour anticorps anti-CD36 (ABIN2191823)

Bastie, Nahlé, McLoughlin, Esser, Zhang, Unterman, Abumrad: "FoxO1 stimulates fatty acid uptake and oxidation in muscle cells through CD36-dependent and -independent mechanisms." dans: The Journal of biological chemistry, Vol. 280, Issue 14, pp. 14222-9, (2005) (PubMed).

Kunjathoor, Febbraio, Podrez, Moore, Andersson, Koehn, Rhee, Silverstein, Hoff, Freeman: "Scavenger receptors class A-I/II and CD36 are the principal receptors responsible for the uptake of modified low density lipoprotein leading to lipid loading in macrophages." dans: The Journal of biological chemistry, Vol. 277, Issue 51, pp. 49982-8, (2002) (PubMed).

Smith, Serghides, Patel, Febbraio, Silverstein, Kain: "CD36-mediated nonopsonic phagocytosis of erythrocytes infected with stage I and IIA gametocytes of Plasmodium falciparum." dans: Infection and immunity, Vol. 71, Issue 1, pp. 393-400, (2002) (PubMed).

Finnemann, Silverstein: "Differential roles of CD36 and alphavbeta5 integrin in photoreceptor phagocytosis by the retinal pigment epithelium." dans: The Journal of experimental medicine, Vol. 194, Issue 9, pp. 1289-98, (2001) (PubMed).

Détails sur CD36

Antigène: CD36

Autre désignation: CD36 (CD36 Produits)

Synonymes: anticorps BDPLT10, anticorps CHDS7, anticorps FAT, anticorps GP3B, anticorps GP4, anticorps GPIV, anticorps PASIV, anticorps SCARB3, anticorps Fat, anticorps Scarb3, anticorps GPIIIB, anticorps PAS-4, anticorps zgc:92513, anticorps CD36 molecule, anticorps CD36 molecule (thrombospondin receptor), anticorps CD36, anticorps Cd36, anticorps cd36

Sujet: Monoclonal antibody CRF D-2712 (also known as JC63.1 or 63) reacts with mouse CD36 (85- kDa), a cell surface class B scavenger receptor. Recently, CD36 has been identified as a protein that is required for TLR2 recognition of di-acylated bacterial lipopeptides and lipoteichoic acid. CD36 is a heavily N- glycosylated transmembrane protein with two short intracellular domains and a large extracellular domain. CD36 expression is primarily controlled by the transcription heterodimer PPARg-RXR (peroxisome proliferator-activated receptor-g-retinoid-X-receptor). CD36 is preferentially found within lipid rafts, which facilitates its association with receptors, signalling and adaptor molecules. CD36 is expressed on monocytes, macrophages, endothelial cells, hepatocytes, erythrocytes, adipocytes, cardiomyocytes and red skeletal muscle. Importantly it is expressed by steriodogenic cells in the adrenal gland, testis and ovary. CD36 has been implicated in many biological processes including angiogenesis, phagocytosis, inflammation, and lipid and glucose metabolism. CD36 is associated with Src-family kinases and with the integrins alpha3beta1 and alpha6beta1. It is a receptor and transporter of oxidized lipids and long chain fatty acids. CD36 has been shown to function as phagocytic receptor for apoptotic cells. Several in vivo models support the role of the thrombospondin / CD36 system in angiogenesis and tumor growth. An important role for CD36 has been found in Malaria as major receptor for P. falciparum- infected red blood cells. Many different ligands have been reported to interact with CD36, suggesting that CD36 could recognize a structure-based domain rather than specific contact residues. Monoclonal antibody CRF D-2712 blocks CD36 binding. It is not known whether the antibody is activating or neutralizing. The antibody cross reacts with rat CD36.

Pathways: Signalisation TLR, Peptide Hormone Metabolism, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Hepatitis C, Toll-Like Receptors Cascades, Lipid Metabolism, S100 Proteins