Nitrotyrosine anticorps (Biotin)

N° du produit ABIN2192140

Détail du produit anti-Nitrotyrosine anticorps (Biotin)

Antigène: Nitrotyrosine

Reactivité: Veuillez nous consulter SVP

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Nitrotyrosine est conjugé à/à la Biotin

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoassay (IA)

Stérilité: 0.2 μm filtered

Clone: HM-11

Information d'application

Indications d'application: For immuno histology and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. Warning: keep sterile, do not add sodium azide or any nitric containing preservative since this will affect the antibody!

Restrictions: For Research Use only

Stockage

Buffer: PBS, containing 0.1 % bovine serum albumin.

Stock: 4 °C

Stockage commentaire: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Date de péremption: 12 months

Références pour anticorps anti-Nitrotyrosine (Biotin) (ABIN2192140)

ter Steege, Koster-Kamphuis, van Straaten, Forget, Buurman: "Nitrotyrosine in plasma of celiac disease patients as detected by a new sandwich ELISA." dans: Free radical biology & medicine, Vol. 25, Issue 8, pp. 953-63, (1999) (PubMed).

Kooy, Royall, Ye, Kelly, Beckman: "Evidence for in vivo peroxynitrite production in human acute lung injury." dans: American journal of respiratory and critical care medicine, Vol. 151, Issue 4, pp. 1250-4, (1995) (PubMed).

Beckmann, Ye, Anderson, Chen, Accavitti, Tarpey, White: "Extensive nitration of protein tyrosines in human atherosclerosis detected by immunohistochemistry." dans: Biological chemistry Hoppe-Seyler, Vol. 375, Issue 2, pp. 81-8, (1994) (PubMed).

Beckman, Beckman, Chen, Marshall, Freeman: "Apparent hydroxyl radical production by peroxynitrite: implications for endothelial injury from nitric oxide and superoxide." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 4, pp. 1620-4, (1990) (PubMed).

Détails sur Nitrotyrosine

Antigène: Nitrotyrosine

Abstract: Nitrotyrosine Produits

Classe de substances: Chemical

Sujet: This highly specific monoclonal antibody HM.11 reacts with nitrotyrosine, both with the free amino acid as well as with proteins containing nitrotyrosine. The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic plaques. Nitrotyrosine is formed in tissues in presence of the active metabolite NO and is a stable end product of nitrosylation of tyrosine. Various pathways including the formation of peroxinitrite lead to nitrotyrosine production.