Immunoprecipitation (IP), ELISA, Western Blotting (WB)
Attributs du produit
Concentration Definition: by UV absorbance at 280 nm
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues near the amino terminus of the human Ube2j1 protein.
This affinity purified antibody has been tested for use in ELISA, western blotting and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 35-40 kDa in size corresponding to Ube2j1 protein by western blotting in the appropriate cell lysate or extract.
For Research Use only
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
(Ubiquitin-Conjugating Enzyme E2, J1, U (UBE2J1))
This antibody is designed, produced, and is suitable for Cancer, Immunology and Nuclear Signaling research. Ube2j1 and Ube2j2 are homologs of the yeast ubiquitin-conjugating enzyme UBC6, which catalyzes the covalent attachment of ubiquitin to other proteins. These proteins constitute a distinct family of ubiquitin-conjugating enzymes sharing a conserved non-canonical active site sequence and a C-terminal transmembrane domain. By analogy with yeast UBC6, Ube2j1 and Ube2j2 are localized to the endoplasmic reticulum and seem to function in the selective degradation of misfolded membrane proteins and in general mediation of the stress response. Synonyms: Non-canonical ubiquitin-conjugating enzyme 1, Yeast ubiquitin-conjugating enzyme UBC6 homolog E