SMAD4 anticorps (C-Term)

N° du produit ABIN233840

N° du produit (Fournisseur): 600-401-a41

L’anticorps Lapin Polyclonal anti-SMAD4 a été validé pour WB et ELISA. Il convient pour détecter SMAD4 dans des échantillons de Humain, Souris et Xenopus laevis.

Aperçu rapide pour SMAD4 anticorps (C-Term) (ABIN233840)

Antigène: SMAD4 (SMAD Family Member 4 (SMAD4))

Reactivité: Humain, Souris, Xenopus laevis

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SMAD4 est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-SMAD4 anticorps

Épitope: C-Term

N° du produit (Fournisseur): 600-401-a41

Fournisseur: Rockland

Fonction: SMAD4 Antibody

Réactivité croisée (Details): This affinity purified antibody is directed against human SMAD4 protein.

Attributs du produit: Synonyms: rabbit anti-SMAD4 antibody, SMAD-4, SMAD 4, mothers against decapentaplegic homolog 4 antibody, MAD homolog 4, Mothers against DPP homolog 4, Deletion target in pancreatic carcinoma 4 antibody, SMAD family member 4, DPC 4, DPC4, MADH4, MADH 4

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Stérilité: Sterile filtered

Immunogène:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminus of human SMAD4 protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Information d'application

Indications d'application:

Application Note: This affinity purified antibody has been tested for use in ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 60 kDa in size corresponding to SMAD4 protein by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:15,000 - 1:60,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.2 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur SMAD4

Antigène: SMAD4 (SMAD Family Member 4 (SMAD4))

Autre désignation: SMAD4

Sujet: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. SMAD4 (also known as Mothers against decapentaplegic homolog 4, Mothers against DPP homolog 4, deletion target in pancreatic carcinoma 4 and hSMAD4) is a common mediator of signal transduction by TGF-b (transforming growth factor), but is also involved in cancer development and metastases as a tumor suppressor.  SMAD4 promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription.  SMAD4 may form trimers with receptor-regulated SMAD (R-SMAD) and interacts with ATF2, COPS5, DACH1, MSG1, SKI and TRIM33.  In the absence of ligand SMAD4 is found in the cytoplasm, but when complexed with R-SMAD, translocates to the nucleus.   Defects in SMAD4 are a cause of pancreatic carcinoma and juvenile polyposis syndrome (JPS), a syndrome in which patients are at risk for developing gastrointestinal cancers.

ID gène: 4089, 13603414

UniProt: Q13485

Pathways: Cycle Cellulaire, Chromatin Binding, Autophagy