STAT5A anticorps (pTyr694)

N° du produit ABIN233843

N° du produit (Fournisseur): 600-401-a45

L’anticorps Lapin Polyclonal anti-STAT5A a été validé pour WB, IHC et ELISA. Il convient pour détecter STAT5A dans des échantillons de Humain.

Aperçu rapide pour STAT5A anticorps (pTyr694) (ABIN233843)

Antigène: STAT5A (Signal Transducer and Activator of Transcription 5A (STAT5A))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp STAT5A est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Détail du produit anti-STAT5A anticorps

Épitope: pTyr694

N° du produit (Fournisseur): 600-401-a45

Fournisseur: Rockland

Fonction: Stat5 phospho Y694 Antibody

Réactivité croisée (Details): This antibody is specific for mouse Stat5a protein phosphorylated at Y694.

Attributs du produit: Synonyms: rabbit anti-STAT5 pY694, phosphorylated STAT5 antibody, STAT-5, STAT 5, STAT5A, Signal Transducer and Activator of Transcription 5A antibody, STAT-5A, Transcription factor STAT5A antibody

Purification: This product was affinity purified from monospecific antiserum by immunoaffinity chromatography using phospho-peptide coupled to agarose beads followed by solid phase adsorption against nonphospho-peptide.

Stérilité: Sterile filtered

Immunogène:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y694 of mouse STAT5a protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 20-40 μg/mL

Application Note: This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 91 kDa in size corresponding to phosphorylated Stat5a protein by western blotting in the appropriate cell lysate or extract.  This phospho-specific polyclonal antibody reacts with mouse Stat5a pY694 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:25,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur STAT5A

Antigène: STAT5A (Signal Transducer and Activator of Transcription 5A (STAT5A))

Autre désignation: Stat5a

Sujet: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Signal transducer and activator of transcription 5 (Stat5) belongs to a family of cytoplasmic transcription factors that can be activated (phosphorylated) by a cell surface receptor.  Phosphorylation at Tyr694 is obligatory for Stat5 activation. Stat5 has two isoforms, Stat5a and Stat5b.  Aberrant Stat5 activation has been implicated in the pathogenesis of chronic myelogenous leukemia, prostate and breast cancer and tumor metastasis.  Stat5 is localized in the cytoplasm and upon phosphorylation at Y694 is translocated to the nucleus.

ID gène: 20850, 6755672

UniProt: P42230

Pathways: Signalistation JAK/STAT, Signalisation RTK, Response to Growth Hormone Stimulus, C21-Steroid Hormone Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events, Activated T Cell Proliferation